Lecture Quiz 3 Flashcards
(27 cards)
What type of rate are constitutive enzymes expressed in ?
FIXED rate
What type of proteins are constantly being transcribed and translated into proteins
Constitutive Enzymes
What are other enzymes that are expressed ONLY AS NEEDED?
- repressible enzymes
- inducible enzymes
What is induction?
INDUCIBLE genes turned ON by INDUCERS
Inducers, often substrates are required for what type of pathways?
Catabolic pathways
What is repression
REPRESSIBLE genes turned OFF by REPRESSORS
Repressors are end products of what type of pathways?
ANABOLIC (biosynthetic) pathways
What happens in anabolic pathways?
- when sufficient product is made gene gets turned off
- this regulation often achieved with the OPEERON
Give an example of bacteria that can utilize lactose that require a lag time during which lac operon becomes induced
E. coli
If medium contains glucose (preferred sugar) and lactose, are lac operon induced?
NO, it is NOT induced. the only time it will be induced is when all glucose is used up
So what if glucose and lactose are present, why not metabolize lactose along with glucose?
- genes for glucose metabolism are CONSTITUTIVE
- activating other genes for more pathways requires energy therefore, bacteria CONSERVE energy by USING GLUCOSE FIRST, then any other energy sources
What is mutation?
change in the genetic material
Can mutations be neutral, beneficial or harmful?
YES
What is a mutagen?
agent that causes mutations
What are spontaneous mutations?
occur in the absence of a mutagen
What is base substitution?
point mutation
What is a missense mutation?
- change in one base
- results in change in amino acid
What is nonsense mutation?
- results in a nonsense codon
- stop codon-> protein is going to be short
What is frameshift mutation?
insertion or deletion of one or more nucleotide pairs
What are three types of mutations?
- missense mutation
- nonsense mutation
- frameshift mutation
What causes the formation of ions that can react with nucleotides and the deoxyribose-phosphate backbone?
ionizing radiation (X-RAYS and GAMMA RAYS)
What repairs mutations?
Nucleotide excision
What causes thymine dimers and bump in DNA?
UV radiation
What separates thymine dimers?
Light-repair