Fa Flashcards

1
Q

simplest and most frequently used

A

DFS

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2
Q

for the observation of the motility of trophozoites

A

0.85% NaCl

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3
Q

doesn’t demonstrate glycogen vacuole and nuclei

A

0.85% NaCl

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4
Q

cysts appear glistening and refractile

A

0.85% NaCl

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5
Q

for protozoan cysts and helminth ova
kill the trophozoites and helminth larva

A

D’antonis solution/Lugol’s Iodine

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6
Q

2 structures seen in cyst in D’antonis

A

Chromatodal bars and glycogen vacuole

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7
Q

Uses of coverslip

A
  1. to avoid drying of the preparation
  2. to avoid fogging and contamination of objectives
  3. to avoid rapid loss of the stain
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8
Q

Appropriate for formed or semiformed stool

A

Kts

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9
Q

For Emulsification of stool

A

Distilled water

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10
Q

Clearing agent in kts

A

Glycerin

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11
Q

Stain inkts

A

3% malachite green

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12
Q

Coverslip in kts

A

Cellophane

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13
Q

Clearing time of kts in room temp

A

1 hour

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14
Q

Clearing time of kts in dry incubation

A

20-30mins

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15
Q

Satisfactory for all kinds of helminth eggs

A

Kts

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16
Q

For large scale examination

A

Kts

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17
Q

Unsuitable for protozoan cysts and trophozoites

A

Kts

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18
Q

Detection of small number of parasites not detected using DFS

A

Concentration techniques

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19
Q

Protozoan cysts, helminth eggs and larvae

A

Sedimentation method

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20
Q

Less efficient than flotation: because protozoan cysts and
many eggs may not be seek.

A

Sedimentation method

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21
Q

Concentrate eggs, larvae and cysts of parasites, operculated and schistosomal eggs

A

Formalin-ether technique

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22
Q

Preservative and fixative

A

Formalin

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23
Q

How many minutes in formalin before centrifugation

A
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24
Q

Dissolves lipids and other colloidal material

A

Ether

25
Q

Material can be examined hours or even days after preparation

A

FECT

26
Q

Not applicable for protozoan cysts

A

Acid ether sedimentation

27
Q

we may observe helminth eggs and larvae

A

Acid ether sedimentation

28
Q

Cleraing agent in acid ether sedimentation

A
29
Q

Not recommended because it is time consuming

A

Simple sedimentation

30
Q

For blood only to detect microfilariae

A

Knott concentration technique

31
Q

Helminths eggs and protozoan cysts may either float or sink depending on their specific gravitate

A

Flotation technique

32
Q

Superior than sedimentation

A

Flotation method

33
Q

Helminth eggs, protozoan cysts and larvae specific gravity

A

1.05-1.15

34
Q

Chemical solutions specific gravity

A

1.12 to 1.21

35
Q

Optimal time for examination of flotation method

A

5-20 minutes

36
Q

Zinc sulfate centrifugal flotation technique specific gravity

A

1.18

37
Q

80% effective in detecting eggs and cysts in light infections

A

ZINC SULFATE CENTRIFUGAL FLOTATION TECHNIQUE

38
Q

Hookworm SG

A

1.005

39
Q

Ascaris (fertile) sg

A

1.110

40
Q

Trichuris sg

A

1.150

41
Q

Giardia sg

A

1.060

42
Q

E. Histolytica sg

A

1.070

43
Q

Laboratory test for occult blood

A

Benzidine
Guiaic
Hematest

44
Q

Meat free diet for how many days prior to occult blood test

A

3-5 days

45
Q

False pos or false neg myoglobin in meat

A

False pos

46
Q

False pos or false neg vitamin c

A

False neg

47
Q

blood which is not visible to the naked eye and can only be detected by chemical means only

A

Occult blood

48
Q

due to hemorrhagic disorder, ulcers and contamination

A

Rbc

49
Q

PRESENT IN BACTERIAL OR PARASITIC INFECTION

A

Macrophages

50
Q

indicative of inflammation

A

Wbc

51
Q

Fungi in occult blood

A

Candida spp

52
Q

Pseudoparasite in occult blood

A

Plant cells, pollen grains and spores

53
Q

Increased during inflammation of the lower bowel

A

Epithelial cells

54
Q

Contaminated by urine in occult blood

A

Calcium oxalate and triple phosphate crystls

55
Q

Similar to helminth ova in occult blood

A

Plant fibers
Root hair
Animal cells

56
Q

Mistaken by trichuris trichuria or karvae

A

Charcot latyden crystals

57
Q

Color of glycogen vacuole

A

Mahigany brown

58
Q

Uses specific gravity and centrifugation

A

Sedimentation method