FASTIDIOUS GNR Flashcards
1
Q
OXIDASE - CATALASE + UREASE - INDOLE - NITRATE + BHI agar- STAR SHAPED AREAS ON COLONIES
A
Actinobacillus actinomycetemcomitans
2
Q
oxidase - catalase + G, MF TM + BCYE + no growth on blood Small yellow green growth Bipolar staining
A
Francisella tularensis
3
Q
oxidase - catalase - growth on blood Rats fried egg appearance
A
streptobacillus
4
Q
oxidase - catalase - no growth on blood urease + capnophilic
A
Ureaplasma urealyticum
5
Q
oxidase - catalase - no growth BAP urease - arginine + fried eggs appearance
A
Mycoplasma hominis
6
Q
oxidase - catalase - no growth on BAP urease - arginine -
A
Mycoplasma pneumoniae
7
Q
oxidase + no growth on BAP Bordet Gengou (drops of mercury) Regan Lowe (mother of pearl) beta hemolytic Urea +
A
Bordetella pertussis
8
Q
oxidase + catalase + gelatinase + motile no growth on BAP L-cysteine BCYE ground glass appearance
A
Legionella pneumophila
9
Q
OXIDASE + no growth on BAP chocolate agar growth require X factor only (hemin) catalase - ALA-porph -
A
Haemophilus ducreyi
10
Q
OXIDASE + no growth on BAP chocolate agar growth req V factor only (NAD) ALA porph + catalase -
A
Haemophilus paraphrophilus
11
Q
OXIDASE + no growth on BAP chocolate agar growth Req V only ALA proph + catalase + gamma hemolysis
A
Haemophilus parainfluenzae
12
Q
OXIDASE + no growth on BAP chocolate agar growth Req V only ALA proph + catalase + beta hemolysis rabbit/ horse blood
A
Haemophilus parahaemolyticus
13
Q
OXIDASE + no growth on BAP chocolate agar growth Req X & V ALA proph - beta hemolysis
A
Haemophilus Haemolyticus
14
Q
OXIDASE + no growth on BAP chocolate agar growth 5-10% CO2 Req X & V ALA proph - catalase + gamma hemolysis
A
Haemophilus influenzae
15
Q
growth on BAP (48-72hr) catalse - indole - Oxidase + Nitrate +
A
Eikenella
16
Q
what organism pits the agar, has a bleach odor, and is common in IV drug users?
A
Eikenella
17
Q
growth on BAP (48-72hr) catalse - lactose fermenter ONPG + ALA-porpho +
A
Haemophilus aphrophilus
18
Q
growth on BAP (48-72hr) oxidase + catalase - NLF ONPG - slow indole + nitrate - capnophilic
A
Cardiobacterium hominis
19
Q
growth on BAP beta hemolytic (48-72hr) GF oxidase + catalase - NLF indole - H2S - can grow on Neisseria media
A
Kingella
20
Q
what are the members of the HACEK group?
A
Haemophilus Actinobacillus / aggregatibacter Cardiobacterium Eikenella Kingella
21
Q
what condition are the HACEK group commonly the cause of?
A
endocarditis
22
Q
oxidase + growth on BAP (48-72hr) catalase + MAC - HE - BCYE + urease + intracellular
A
Brucella spp
23
Q
oxidase + growth on BAP (48-72hr) catalase + extracellular safety pin appearance indole + ornithine + capnophilic
A
Pasteurella multocida
24
Q
what organisms require cholesterol in order to grow?
A
Mycoplasma
Ureaplasma
25
```
which Brucella spp matches these reactions:
CO2 +
H2S +
Thionine +
basic fuschin -
```
abortus (cow)
26
```
which Brucella spp matches these reactions:
CO2 -
H2S +/-
Thionine -
basic fuschin +
```
suis (pigs)
27
```
which Brucella spp matches these reactions:
CO2 -
H2S -
thionine -
basic fuschin -
```
melentensis (goats)
28
what is the mode of transmission for F. tularensis?
tick bite
29
what are the environmental factors required for fastidiuous organisms to grow?
5 - 10% CO2
30
```
Catalase -
oxidase -
hippurate +
starch +
10% KOH = fishy odor
Diffuse hemolysis on human blood plates
Requires increased CO2 48 - 72 hr incubation
```
gardnerella vaginalis
31
what organism will grow in hemolytic zones around staph?
haemophilus
32
```
oxidase -
catalase -
indole -
gliding motility
Increased CO2
Ferment g/l/s
```
capnocytophaga
33
what organism causes a positive cold agglutinin titer?
M. pneumoniae
34
Normal flora nonpathogenic haemophilus spp
Parainfluenzae
Haemolyticus
Parahaemolyticus
35
H ducreyi infections
Chancroid
36
Bordetella pertussis infections
Whooping cough
37
Brucellosis infections
Brucellosis (undulant fever)
38
Brucellosis transmission
Unpasteurized milk
| Contact with infected goats, cows, hogs dogs
39
Francisella infections
Tularemia
40
Francisella transmission
Tick or infected animal
41
Gardernella infections
```
Bacterial vaginosis (no lactobacillus)
Bacteremia
```
42
Legionella infection
Legionnaires disease
| Pontiac fever
43
Legionella transmission
Found in water
| Inhalation of aerosol
44
Pasteurella infections
Wound and respiratory tract infections
45
Pasteurella transmission
Bite or scratch of cat or dog
46
What reactions can be expected when para is in the species name for haemophilus
Require V factor
| ALA +
47
What reaction is expected on rabbit and horse blood agar with haemolyticus spp of haemophilus
Hemolysis
48
Medium of choice for francisella tularensis
Cysteine blood agar
49
factor X
hemin
50
factor V
NAD
51
why do haemophilus species that require X and V factors grow around staph species on BAP?
staph synthesizes NAD and hemin is released upon beta hemolysis
52
X and V
| no hemolysis
influenzae
| aegyptius
53
V factor
| no hemolysis
parainfluenzae
54
X and V
| hemolysis
haemolyticus
55
V factor
| hemolysis
parahemolyticus
56
X factor only
ducreyi
57
methods to detect legionella
culture
DFA
serological methods
58
pertussis swab collection
dacron
59
obligate parasites
bordetella
brucella
bartonella
60
brucella agar ingredients
casein
animal tissue
yeast
sodium bisulfite
61
what is the special media required for francisella?
blood cysteine glucose agar with thiamine
62
streptobacillus infection
rat bite fever
| Haverhill fever
63
HACEK group characteristics
colonize oropharynx
| enhanced growth under increased CO2
64
differentiate Neisseria and Kingella
N- Catalase +
| K- catalase -
65
organisms associated with animals
brucella
pasteurella
capnocytophaga
francisella
66
H. influenzae disease
meningitis
epiglottitis
penumonia <5 >60
pink eye
67
school of fish
H ducreyi
68
bartonella infections
cat scratch fever
trench fever
carrions disease
69
Differentiate h aegyptius and h influenzae
A - indole and xylose -
| I- indole and xylose +
70
How long does Legionella media need to be incubated?
At least 7 days
