Media

Question 1

What is the purpose of MAC?

Answer 1

Selective and differential for G-

Pink - LF
Yellow/clear - NLF

Question 2

What is the function of EMB?

Answer 2

Differentiate LF and NLF
LF - purple / green metallic sheen
NLF - colorless

Question 3

What is the function of Hektoen Enteric?

Answer 3

Selects for G- enterics 
Differentiate salmonella and shigella
Salmonella - H2S black center
Shigella- clear/green (pathogen) 
Yellow - nonpathogen

Question 4

What is the function of XLD agar?

Answer 4

Select for G- enterics
Differentiate salmonella and shigella

Salmonella- red with H2S black center
Shigella - clear pink to red

Question 5

what is the selective agent in MSA?

Answer 5

7.5 - 10% salt, carbohydrate-alcohol mannitol, phenol red

Question 6

what does MSA select for?

Answer 6

staph species

Question 7

what does it mean when the MSA turns yellow?

Answer 7

the organism ferments mannitol and created an acidic end product

Question 8

what is the selective agen in CNA?

Answer 8

antibiotics: colistin and nalidixic acid

Question 9

what does CNA inhibit?

Answer 9

G- bacteria

Question 10

what is the purpose of TCBS agar?

Answer 10

to select for Vibrio spp and differentiate V cholerae and other spp

Question 11

what does a positive TCBS look like?

Answer 11

yellow colonies (V. cholerae)

Question 12

what are the inhibitors in MAC?

Answer 12

bile salts and crystal violet

Question 13

what are the selective agents in Modified Thayer Martin?

Answer 13

vancomycin
colistin- G-
nystatin
trimethoprim

Question 14

what is New york City media made out of?

Answer 14

lysed horse blood
horse plasma
yeast dialysate
antibiotics

Question 15

what media is used for the isolation of Bordetella pertussis?

Answer 15

Bordet-gengou

Regan Lowe

Question 16

what is CIN agar used for?

Answer 16

Yersinia

Question 17

what is Martin Lewis media used for?

Answer 17

isolation of N. gonorrhoeae

Question 18

what media is used for Mycobacterium?

Answer 18

middlebrook

petraganis

Question 19

what supplement is added to BBA?

Answer 19

vitamin K1

hemin

Question 20

what is BLKV agar used for?

Answer 20

isolate GNR obligate anaerobes, esp bacteroides, prevotella, fusobacterium, veillonella

Question 21

what is CBA used for?

Answer 21

aerotolerance testing

Question 22

what is BBE selective for?

Answer 22

B fragilis

Question 23

what is PEA agar used for?

Answer 23

isolation of G+ and G- obligate anaerobes and G+ facultative anaerobes

Question 24

what is egg yolk agar used for?

Answer 24

detection of lectithinase lipase and proteolysis for clostridium

Question 25

what is cooked meat agar used for?

Answer 25

clostridium

Question 26

what is Todd Hewitt broth used for?

Answer 26

grow strep for serotyping

Question 27

what agars are used for Mycobacterium spp?

Answer 27

Lowenstein Jensen Middlebrook 7H10 Petragnani

Question 28

what is anaerobic blood agar enriched with?

Answer 28

hemin cystine vitamin K yeast

Question 29

what is BCYE agar used for?

Answer 29

Legionella

Question 30

what is Fletchers medium used for?

Answer 30

Leptospira

Question 31

what agars are used for Corynebacterium?

Answer 31

Tinsdale | Loefflers

Question 32

bile esculin agar purpose

Answer 32

selective for enterococcus spp

Question 33

CCFA agar purpose

Answer 33

selective for C. difficile (yellow colonies)

Question 34

organism requires X and V factors

Answer 34

haemophilus

Question 35

What does a yellow colony on TCBS mean?

Answer 35

Ferments sucrose

Question 36

Yellow colonies on CCFA mean what

Answer 36

Fermentation of fructose

Question 37

Media used for Neisseria gonorrhoeae

Answer 37

MTM New York City Martin Lewis

Question 38

DCA purpose

Answer 38

Inhibits gram positive organisms and most enterics | isolation of salmonella and shigella

Question 39

DCA inhibitory factors

Answer 39

Citrate | Deoxycholate salts

Question 40

DCA Differentiates what?

Answer 40

LF- pink | NLF - colorless

Question 41

Cetrimide selective for

Answer 41

P aeruginosa

Question 42

What do yellow colonies on HE agar mean?

Answer 42

Sucrose fermentation

Question 43

CTA media purpose

Answer 43

Growth of Neisseria spp

Question 44

What affects the redox potential of anaerobic media

Answer 44

pH

Question 45

Broth used for anaerobic media to detect volatile fatty acids

Answer 45

PYG