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Hematology Module 1 and 2 > Flow Questions > Flashcards

Flashcards in Flow Questions Deck (20):
1

What are the three applications of cytometry?

Diagnosis and subclassifications of leukemias and lymphomas. (2) Detection on minimal residual disease before relapse (3) additional info in challenging cases

2

Brief flow definition.

Fluorescent Monoclonal antibodies to antigens expressed at specific stages of normal hematopoietic maturation. Single file and shot at with laser and then detected.

3

Define hydrodynamic focusing

makes flow cytometer more accurate for determining size

4

Define gating

selection of a certain population

5

Define data acquisition gating

Comprehensive analysis of all cells, internal positive and negative controls, detection of unexpected abnormal populations

6

Define live gating

Selection of a certain cell population

7

Forward scatter?

The size of a cell

8

Side scatter?

The complexity of the cytoplasm

9

Look at normal flow scatter

Page 17 PPT one

10

Immature cell marker?

34, 117, TdT

11

Granulocytic/monocytic marker?

34, 33, 15, 13, 14

12

Erythroid precursor marker?

71, glycophorin A

13

Megakarocytes marker?

41,42,61

14

B-Cell marker?

19,20 Kappa/Lambda light chains

15

T cell markers?

2,5,7,4,8

16

What does CD stand for?

Cluster designation or cluster of differentiation

17

What marker is on all cells?

CD 45

18

Where are the sites of production for CLL and SLL?

CLL is BM, SLL is lymph nodes

19

How does a germinal center differ from a follicle?

Germinal centers have been presented with an antigen

20

What describes a lymphoma?

Lymphomas involve lymphocytes and typically present in lymph nodes