Gluconeogenesis, HMP Shunt Flashcards Preview

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Flashcards in Gluconeogenesis, HMP Shunt Deck (88)
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1

Gluconeogenesis irreversible enzymes

1. Pyruvate carboxylase
2. Phosphoenoyruvate carboxykinase
3. Fructose-1,6-bisphosphatase
4. Glucose-6-phosphate

2

Pyruvate carboxylase reaction and location

Pyruvate + CO2+ATP --> oxaloacetate
Mitochondia

2

Pyruvate carboxylase is activated by

Acetyl coa

3

Pyruvate carboxylase requirement

Biotin
ATP

4

Phosphoenolpyruvate carboxykinase requirements

GTP

5

Phosphoenolpyruvate carboxykinase reaction and location

Oxaloacetate to phosphoenolpyruvate
cytosol

7

Fructose -1,6- bisphosphatase reaction and location

Fructose -1,6-BP-->fructose-6-P
cytosol

8

Fructose -1,6- bisphosphatase regulation

Citrate+
Fructose 2,6-BP-
AMP -
ATP +

8

Glucose-6-phosphatase reaction

Glucose -6-P-->glucose

9

Glucose-6-phosphatase LOCATION / AND ORGAN

ENDOPLASMIC RETICULUM
PRIMARILY IN LIVER

10

Gluconeogenesis serves

To maintain euglycemia during fasting

11

Gluconeogenesis tissues

Liver(primary)
Kidney
Intestinal epithelium

12

Deficiency of key gluconeogenic enzymes cause

Hypoglycemia

13

Muscle - gluconeogenesis

no --> lacks glucose -6 phosphatase

15

fatty acids/gouconeogenesis

Even chain fatty acids --> cannot produce new, since they yield only acetyl-CoA equivalents
Odd-chain fatty acids --> yield one propionyl-CoA during metabolism, which can enter TCA (as succinyl-CoA), undergo gluconeogenesis

15

Propionyl-CoA can enter TCA cycle as

Succinyl - CoA

16

Odd-chain fatty acids/gluconeogenesis

They yield one propionyl-CoA during metabolism, which can enter TCA (as succinyl-CoA), undergo gluconeogenesis

17

Source of NADPH

HMP shunt

18

HMP

Pentose phosphate pathway

19

HMP provides a source of

NADPH

20

HMP provides a source of from abundantly available

Glucose-6-P

21

HMP yields

1. NADPH
2. Ribose for nucleotide synthesis
3. Glycolytic intermediates

22

How many pathways for HMP shunt

2.
Oxidative and nonoxidative

23

NADPH function

1. Glutathione reductase
2. Cytochrome P-450
3. Respiratory burst
4. Anabolic process (steroid and farry acids synthesis)

24

Location of of oxidative HMP shunt

Cytoplasm

25

Location of nonoxidative HMP shunt

Cytoplasm

26

ATP/HMP shunt

NO ATP IS USED OR PRODUCED IN HMP SHUNT

27

Is oxidative HMP shunt reversible or irreversible

Irreversible

28

Sites of HMP shunt (organs)

Sites of fatty acid or steroid synthesis (lactating mammary glands, liver, adrenal glands), RBCs

29

Is nonoxidative reaction reversible or irreversible

Reversible