Haemoflagellates/Leishmania Flashcards Preview

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Flashcards in Haemoflagellates/Leishmania Deck (16)
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1

Haemoflagellates

~ parasitic eukaryotic protists
~ all vector borne
~ all have flagellum at some point

2

Name the three main important haemoflagellates.

Leishmania
Trypanosoma brucei
Trypanosoma cruzi

3

Name the three forms of Leishmaniasis.

~ cutaneous - skin
~ mucocutaneous - around mucus e.g. nose
~ visceral - spleen, liver, high death rate

4

Canine visceral leishmaniasis

zoonotic disease caused by Leishmania infant
~ domestic dog main reservoir host

5

Name the methods for cutaneous leishmaniasis diagnosis.

tissue sample for smear and culture --> microscopy

6

Name the methods for visceral leishmaniasis diagnosis.

~ Bone marrow biopsy or splenic aspirate --> smear & culture --> microscopy
~ Skin test
~ IFAT
~ ELISA
~ PCR

7

PCR

~ allows amplification of small DNA sample
~ only specific DNA samples are replicated due to spp/genus specific primer

8

ELISA

a plate assay technique that detects and qualifies substances through binding them to antibody conjugates

9

Name the three types of ELISA.

1. direct
2. indirect
3. sandwich

10

Direct ELISA

substrate binds to bound primary antibody conjugate

11

Indirect ELISA

substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate

12

Sandwich ELISA

substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate which is bound to a capture antibody

13

Immunochromatographic rK39 test

rapid urine dipstick test that identifies the Leishmania antigen rK39 using recombinant protein K39

14

Direct agglutination test (DAT)

~ combination of insoluble antigen and soluble antibody = agglutination
~ serial diltution performed on sample --> semi quantitative

15

Fast agglutination screening test (FAST)

~ modified DAT
~ detected antibodies rK26/rK39
~ quicker

16

DDP

~ innovative colloidal gold-based immunochromatography assay
~ rapid (5-10mins)