Flashcards in Haemoflagellates/Leishmania Deck (16)
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1
Haemoflagellates
~ parasitic eukaryotic protists
~ all vector borne
~ all have flagellum at some point
2
Name the three main important haemoflagellates.
Leishmania
Trypanosoma brucei
Trypanosoma cruzi
3
Name the three forms of Leishmaniasis.
~ cutaneous - skin
~ mucocutaneous - around mucus e.g. nose
~ visceral - spleen, liver, high death rate
4
Canine visceral leishmaniasis
zoonotic disease caused by Leishmania infant
~ domestic dog main reservoir host
5
Name the methods for cutaneous leishmaniasis diagnosis.
tissue sample for smear and culture --> microscopy
6
Name the methods for visceral leishmaniasis diagnosis.
~ Bone marrow biopsy or splenic aspirate --> smear & culture --> microscopy
~ Skin test
~ IFAT
~ ELISA
~ PCR
7
PCR
~ allows amplification of small DNA sample
~ only specific DNA samples are replicated due to spp/genus specific primer
8
ELISA
a plate assay technique that detects and qualifies substances through binding them to antibody conjugates
9
Name the three types of ELISA.
1. direct
2. indirect
3. sandwich
10
Direct ELISA
substrate binds to bound primary antibody conjugate
11
Indirect ELISA
substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate
12
Sandwich ELISA
substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate which is bound to a capture antibody
13
Immunochromatographic rK39 test
rapid urine dipstick test that identifies the Leishmania antigen rK39 using recombinant protein K39
14
Direct agglutination test (DAT)
~ combination of insoluble antigen and soluble antibody = agglutination
~ serial diltution performed on sample --> semi quantitative
15
Fast agglutination screening test (FAST)
~ modified DAT
~ detected antibodies rK26/rK39
~ quicker
16
