Flashcards in Haemoflagellates/Leishmania Deck (16)
~ parasitic eukaryotic protists
~ all vector borne
~ all have flagellum at some point
Name the three main important haemoflagellates.
Name the three forms of Leishmaniasis.
~ cutaneous - skin
~ mucocutaneous - around mucus e.g. nose
~ visceral - spleen, liver, high death rate
Canine visceral leishmaniasis
zoonotic disease caused by Leishmania infant
~ domestic dog main reservoir host
Name the methods for cutaneous leishmaniasis diagnosis.
tissue sample for smear and culture --> microscopy
Name the methods for visceral leishmaniasis diagnosis.
~ Bone marrow biopsy or splenic aspirate --> smear & culture --> microscopy
~ Skin test
~ allows amplification of small DNA sample
~ only specific DNA samples are replicated due to spp/genus specific primer
a plate assay technique that detects and qualifies substances through binding them to antibody conjugates
Name the three types of ELISA.
substrate binds to bound primary antibody conjugate
substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate
substrate binds to bound secondary antibody conjugate which is bound to primary antibody conjugate which is bound to a capture antibody
Immunochromatographic rK39 test
rapid urine dipstick test that identifies the Leishmania antigen rK39 using recombinant protein K39
Direct agglutination test (DAT)
~ combination of insoluble antigen and soluble antibody = agglutination
~ serial diltution performed on sample --> semi quantitative
Fast agglutination screening test (FAST)
~ modified DAT
~ detected antibodies rK26/rK39