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Flashcards in hIGH THROUGHOUT SCREENING Deck (8):
1

•How do antibiotics work?

Bactericidal vs bacteriostatic

Target cell wall, dna synthesis, protein synthesis, cell membrane

2

Antibiotic resistance factors

efflux

immunity and bypass
target modifications
inactivativing enzymes

3

Conjugation

transfer of genetic material between bacterial cells of same or different species by direct cell-to-cell contact.

4

WHO Priority Pathogens

campylobacter
salmonella species
shigella spp
staphylococcis aureus

5

Antibacterial drug discovery Main approaches

Target-based design
Whole cell screening

6

Target-based design

Pick target Design inhibitors screen inhibitor

7

Ethical Responsibilities

Replacement RefinementReduction

8

Whole-cell screening –

screen for inhibitors against whole bacterial cells. Advantage: if get activity, know it is active against organism. Disadvantage: if active, don’t know mechanism of action/target