Flashcards in Intro Deck (24)
collections of cells having similar morphological characteristics
cells that cover surfaces, line body cavities or form solid glands
cells with contractile properties
cells forming the brain, spinal cord and nerves
wide range of living material characterized by dominant extracellular matrix component and associated cells that produce this. Acts as supporting stroma serving more highly specialised cell types.
includes bone, cartilage, tendon, fibrous tissue, adipose tissue, bone marrow & blood.
contains cells of same structure and function.
contains mixture of cells with different functions eg. nervous tissue made up of nerve cells (neurons), support cells (astrocytes), immune cells (microglia) and epithelial cells (ependyma).
anatomically distinct group of tissues (several types) which perform specific functions.
cells with similar function but widely distributed in several anatomical sites;
group of organs that have similar/related functional roles.
-Tissue mounted on glass slide as thin preparation
-Stained with appropriate dye
-Illuminated by light
-Viewed using glass lens
Histology, or histopathology if biopsy from diseased tissue being studied.
analysis of fine structure of cells by light microscopy.
beam of electrons fired at specimen
greatly increases resolution compared to light microscopy.
subcellular composition of cells defined.
antibodies are applied to specific cell constituents to visualise details within cells at the light-microscopic level that aren't visible by other techniques.
What is the most commonly used dye/stain?
Haematoxylin and Eoisin
What colour does H&E (Haematoxylin) stain nuclei?
What colour does H&E (Eosin) stain cytoplasm and extracellular fibres (eg. collagen/elastic)?
Which stain is used to see sugars?
PAS (Periodic acid schiff)
Stains sugars magenta
What is PAS therefore useful for looking at?
Goblet cells in SI and glycosaminoglycans in intestinal brush border.
Which stain is used to see elastic tissue?
Stains elastic brown
Which stain is used to see mucins?
Stains mucin blue
Which stain is used to stain a variety of tissues different colours in the same section?
Give 6 examples of cell shapes:
Rounded eg. RBC
Fusiform (spindle shaped eg. smooth muscle)
Cuboidal (as tall as they are wide, roughly square in 2D eg. thyroid)
Columnar (taller than they are wide)
In general, which cells are smaller out of metabolically active/inactive cells?