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Flashcards in Intro Deck (24)
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1

Tissues

collections of cells having similar morphological characteristics

2

Epithelial tissue

cells that cover surfaces, line body cavities or form solid glands

3

Muscular tissue

cells with contractile properties

4

Nervous tissues

cells forming the brain, spinal cord and nerves

5

Connective tissue

wide range of living material characterized by dominant extracellular matrix component and associated cells that produce this. Acts as supporting stroma serving more highly specialised cell types.
includes bone, cartilage, tendon, fibrous tissue, adipose tissue, bone marrow & blood.

6

Simple tissue

contains cells of same structure and function.

7

Compound tissue

contains mixture of cells with different functions eg. nervous tissue made up of nerve cells (neurons), support cells (astrocytes), immune cells (microglia) and epithelial cells (ependyma).

8

organ

anatomically distinct group of tissues (several types) which perform specific functions.

9

system

cells with similar function but widely distributed in several anatomical sites;
group of organs that have similar/related functional roles.

10

Light microscopy

-Tissue mounted on glass slide as thin preparation
-Stained with appropriate dye
-Illuminated by light
-Viewed using glass lens

Histology, or histopathology if biopsy from diseased tissue being studied.

11

Cytology

analysis of fine structure of cells by light microscopy.

12

Electron microscopy

beam of electrons fired at specimen
greatly increases resolution compared to light microscopy.
subcellular composition of cells defined.

13

Immunohistochemical methods

antibodies are applied to specific cell constituents to visualise details within cells at the light-microscopic level that aren't visible by other techniques.

14

What is the most commonly used dye/stain?

Haematoxylin and Eoisin

15

What colour does H&E (Haematoxylin) stain nuclei?

Blue

16

What colour does H&E (Eosin) stain cytoplasm and extracellular fibres (eg. collagen/elastic)?

Pink

17

Which stain is used to see sugars?

PAS (Periodic acid schiff)

Stains sugars magenta

18

What is PAS therefore useful for looking at?

Goblet cells in SI and glycosaminoglycans in intestinal brush border.

19

Which stain is used to see elastic tissue?

Van Gieson

Stains elastic brown

20

Which stain is used to see mucins?

Alcian Blue

Stains mucin blue

21

Which stain is used to stain a variety of tissues different colours in the same section?

Trichrome stains

22

Give 6 examples of cell shapes:

Rounded eg. RBC
Polygonal (irregular)
Fusiform (spindle shaped eg. smooth muscle)
Squamous (flattened)
Cuboidal (as tall as they are wide, roughly square in 2D eg. thyroid)
Columnar (taller than they are wide)

23

In general, which cells are smaller out of metabolically active/inactive cells?

Inactive

24

................ active cells often have nucleoli

Metabolically