Intro to histology Flashcards
histology
microscopic study of normal cells and tissues
what are examples of physical and chemical insults that can affect tissue architecture
infection extreme temperatures ageing, wear and tear cancers etc
light microscopy
tissues illuminated by a beam of light, gives a larger view and reveals basic structure
electron microscopy
tissues illuminated by a beam of electrons , reveals cell ultrastructure but in a smaller view
what are the 7 steps to obtaining tissues for microscopy
1 - specimen collection 2 - fixation 3 - dehydration 4 - embedding 5 - sectioning 6 - staining 7 - viewing
punch biopsy
used to obtain specimen from skin or oral surfaces
needle biopsy
used to obtain specimens from organs or lumps below the skin
often used alongside X-ray/ MRI to guide biopsy
endoscopic biopsy
flexible tube inserted into incision of opening.
what is a common fixative
formaldehyde
why are samples dehydrated during tissue collection
most methods of embedding e.g paraffin are not compatible with water
microtome
machine used to section embedded specimens
haematoxylin and eosin
most common staining technique
haem - stains acidic structures blue/ purple
eosin - stains basic structures pink/ red
