introductio Flashcards

1
Q

where are the puncture site for infants <1 year during skin puncture

A

plantar surface
medial side of the heel
lateral side of the heel

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2
Q

for older child and adults the site for skin puncture ____

A

palmar surface of the non-dominant hand
(3rd or 4th finger distal portion)

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3
Q

recommended depth of skin puncture for infants?

A

<2.0mm

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4
Q

recommended depth of skin puncture for adults and older children.

A

2.0-2.5 mm

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5
Q

what are the possible effects of squeezing, milking the site?

A

cause hemolysis and introduce to excess interstitial fluid

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6
Q

why do we need to discard the 1st drop of blood?

A

to discard excess tissue fluid
to discard dead epidermal cells
to facilitate free flow of f blood

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7
Q

order of draw for skin puncture

A

tube for blood gas analysis
slides
EDTA microcollection tube
other microcollection tube with anticoagulant
serum micro collection tube

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8
Q

what is the 1st tube collected from skin puncture?

A

blood gas

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9
Q

what is the 1st micrrocollection tube to be collected in skin puncture?

A

EDTA

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10
Q

MOST COMMON SITE FOR VENPUNCTURE

A

SUPERFICIAL VENIS ON THE ANTECUBITAL FOSSA

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11
Q

CLEANSE THE SITE USING __ SCRUB WITH MOVEMENT _____

A

FRICTION
BACK AND FORTH

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12
Q

VEINS TO BE USED IN ORDER OF PREFERENCES
H PATTERN

A

MEIDAN CUBITAL VEIN
CEPHALIC VEIN
BASILIC VEIN

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13
Q

VEINS TO BE USED IN ORDER OF PREFERENCE FORR M PATTER

A

MEDIAN VEIN
ACCESSORY CEPHALIC VEIN
BASILIC VEIN

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14
Q

ANGLE BETWEEN SKIN AND NEEDLE

A

<30 DEGREE
OR 7.5-10 CM

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15
Q

WHAT IS THE LENGTH OF TIME FOR TOURNIQUET APPLICATION

A

LESS THAN 1 MINUTE

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16
Q

DISTANCE OF TOURNIQUET

A

3-4 INCHES

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17
Q

EFFECTS OF PROLONGEED TOURNIQUET APPLICATION

A

HEMOLYSIS
HEMOCONCENTRATION
SHORTENED COOAGULATION TIME

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18
Q

SOME SITE TO AVOID DURING VENIPUNCTURE

A

VEINS IN THE INNER WRIST
VEINS OF THE FEET
FISTULA
ARTERIES
Inflammous SITE
EDEMATOUS SITE

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19
Q

SOME CAUSE OF SPX HEMOLYSIS

A

PROLONGED TOURNIQUET APPLICATION
MOISTURE/CONTAMINATION IN THE BLOOD COLLETION TUBE
USING NEEDLE WITH TOO SMALL. BORE
EXCESSIVE AGITATIOM
FROTHING OF THE BLOOD SAMPLE

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20
Q

MOST COMMON. SKIN ANTISEPTIC

A

70% ISOPROPYL ALCOHOL

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21
Q

MOST COMMON NEEDLE SIZE FOR ADULTS
AND LENGTH

A

21 G
LENGTH OF 1 INCH

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22
Q

MOST COMMON CAUSE OF A NEEDLE PUNCTURE FROM OTHER SHARP OBJECT IS ____

A

IMPROPER DISPOSAL

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23
Q

MOST COMMONLY USED BLOD COLLECTION TUBE IN THE HEMA SECTION

A

EDTA (LAVENDER/PURPLE TOP)

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24
Q

ANTICOAGULANT USED FOR HEMA DETERMINATION

A

K2 EDTA

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25
Q

CBC IS ALSO CALLED

A

HEMOGRAM

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26
Q

POSSIBLE CAUSES OF CLOTTED BLOOD SPECIMEN

A

DIFFICULT PHLEBOTOMY
BLOD SAMPLE WAS NOT INVETED IN THE CORRECT NUMBER OF TIMES
EEXPIRED BLOOD TUBE
OVERFILLED TUBE

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27
Q

OPTIMAL ANTICOAGULANT CONCENTRATION IS FOR EDTA

A

1.5 MG PER ML OF BLOOD

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28
Q

BLOOD SPECIMENS FOR CBC MUST BE ANALYZED WITHIN (IF STORED IN ROOM TEMP)

A

6 HRS OF COLLECTION

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29
Q

BLOOD SPECIMENS FORR CBC MUST BE ANALYZED WITHIN __ (STORED AT 4C)

A

24 HOURS

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30
Q

PREFFERED ANTICOAGULANT FOR PLATELET COUNT

A

EDTA

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31
Q

BLOOD. SMEAR SHOULD BE MADE WITHIN

A

3 HOURS OF COLLECTION

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32
Q

WHAT IS THE CAUSE OF INSUFFICIENT EDTA

A

OVER FILLED TUBE

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33
Q

WHAT IS THE EFFECT OF INSUFFICIENT EDTA

A

PRESENCE OF CLOT

34
Q

WHAT IS THE CAUSE OF EXCESSIVE EDTA

A

UNDER-FILLED TUBE

35
Q

WHAT IS THE EFFECT OF EXCESSIVE EDTA

A

FALSE LOW HCT, ESR,
DEGENERATIVE WBC
FALSE HIGH MCHC AND PLATELET COUNT

36
Q

FORMATION OF RING. SHAPED MOLECULAR COMPLEX N SHICH METAL ION IS COVALENTLY BOUND

A

CHELATION

37
Q

A PROCESS WHICH A CURRENT TEST RESULT IS COMPARED WITH THE RESULT OF THE SAME TEST FROM THE PREVIOUS SPECIMEN FROM THE SAME PATIENT

A

DELTA CHECK

38
Q

TEST BEING ORDERED AUTOMATICALLY BASED ON THE RESULT PRIOR TEST PF [RESET PARAMETERS

A

REFLEX TEST

39
Q

ANY NUMERICAL VALUE THAT DEPICTS AN ENTIRE POPULATION

A

PARAMETER

40
Q

USED FOR FLOW CYTOMETRY
PLASMA CHEM DETECTION
OSMOTIC FRAGILITY TEST

A

GREEN TOP (HEPARIN)

41
Q

OPTIMAL ANTICOAGULANT CONCENTRATION OF HEPARIN IS

A

15-20. UNITS PER ML OF BLOOD

42
Q

HEPARIN IS ANTICOAGULANT OF CHOICE FOR

A

BLOOD GAS ANALYSIS
OSMOTIC FRAGILITY TEST

43
Q

CAUSE THE LEAST INTERFERENCE IN CHEMISTRY TESTING

A

LITHIUM HEPARIN

44
Q

MOST WIDELY USED ANTICOAGULANT FOR PLASMA AND WHOLEE BLOOD CHEM

A

LITHIUM HEPARIN

45
Q

USED FOR COAG TEST

A

BLUE TOP (3.2% SODIUM CITRATE)

46
Q

HEPARIN CAN CUASE CELLULAR CLUMPING WHICH LEAD TO:

A

[SEUDOLEUKOCYTOSIS AND PSEUDOTHROMBOCYTOPENIA

47
Q

CRITICAL RATIO OF BETWEEN 3.2% SODIUM CITRATE AND BLOOD:

A

1:9.

48
Q

WHAT WILL BE THE CLOTTING TIMES YOU HAPPEN TO FORCEFUL MIXING OR NUMBER OF INVERSION

A

SHORTEN CLOTTING TIME

49
Q

ORDER OF DRAW FOR VENIPUNCTURE

A

BLOOD CULTURE TUBE (YELLOW)
CITRATE TUBE (LIGHT BLUE TOP)
SERUM TUBE (RED WITH OR WITHOUT ADDITIVE)
HEPARINIZED TUBE (GREEN)
EDTA (LAVENDER)
SODIUM FLUORIDE (GRAY)

50
Q

NO OF INVERSION FOR YELLOW TOP C

A

8

51
Q

NO OF INVERSION FOR LIGHT BLUE TOP

A

3-4

52
Q

NO OF INVERSION FOR RED TOP (NON ADDITIVE)

A

0

53
Q

NO OF INVERSION FOR RED TOP (PLASTIC HAS CLOT ACTIVATOR)

A

5

54
Q

NO OF INVERSION FOR GREEN TOP

A

8

55
Q

NO OF INVERSION FOR PURPLE TOP

A

8

56
Q

NO OF INVERSION FOR GRAY TOP

A

8

57
Q

WHAT BLOOD COLLECTION TUBES AND ANTICOAGULANT USED FOR LEAD DETERMINATION

A

TAN
K2 EDTA

58
Q

WHAT BLOOD COLLECTION TUBES AND ANTICOAGULANT USED FR TOXICOLOGY, NUTRITIONAL CHEM
TRACE ELEMENT DETECTION

A

ROYAL BLUE TOP
K2 EDTA

59
Q

WHAT BLOOD COLLECTION TUBES AND ANTICOAGULANT USED FOR MOLECULAR DIAGNOSTIC TESTS

A

WHITE TOP
K2 EDTA (WITH GEL)

60
Q

WHAT BLOOD COLLECTION TUBES AND ANTICOAGULANT USED FOR WESTERGREN
ESR

A

BLACK TOP
3.8% SODIUM CITRATE

61
Q

WHAT BLOOD COLLECTION TUBES AND ANTICOAGULANT USED FOR BLOOD BANK TEST AND WHOLE BLOOD HEMA DET.

A

PINK TOP

62
Q

what are the cause of falsely high rbc

A

wbc >100,000, giant platelet cryglobulin, cryofibinogen,`

63
Q

what are the causes of falsely low rbc

A

cold agglutinins, autoagglutination, clotting, microcytsis, schistocytes, hemolysis invitro

64
Q

what are the causes of falsely high MCV

A

cold agglutinin
autoagglutination
high wbc (>50,000)
old specimen
hypersmolar state
reduced RBC deformability

65
Q

what are the causes of falsely low mcv

A

cryoglobulin
cryofibrinogen
hemolysis (in vitro)
swollen rbc
giant platelets

66
Q

what are the causes of falsely high mchc

A

lipemia, icterus, chylomicron, high wbc (>50,000)
spuriously high hb spuriously low rbc

67
Q

what are the causes of falsely low MCH

A

spuriously low hb, falsely high rbc

68
Q

what are the causes of falsely higch mchc

A

cold agglutinin
autoagglutination
hemolysis
clotting
spuriously high hb
falsely low hct

69
Q

what are the causes of falsely low mchc

A

high wbc >50,000
spuriously high hct.
falsely low hb

70
Q

what are the causes of falsely high hemoglobin

A

lipemia,
icterus
chylomicron
lysis resistant rbc with abnormal hb
WBC >20,000
platelet. >700 x109
parenteral nutrition
hypergammaglobulinemia
cyroglobin
cryofibrinogen
heparin
hyperbilirubinemia
hemolysis invitro

71
Q

what are the causes of falsely low hb

A

clotting

72
Q

what are the causes of falsely high hct

A

dehydration
hemoconcentration
iinsufficient centrifugation
buffy coat inclusion
hyponatrema
plasma trapping

73
Q

what are the causes of falsely low hct

A

hemolysis,
improper sealing of capillary tube
increased anticoagulant concentration
introductin of excess tissue fluid
hypernatremia

74
Q

cause of falsely high WBC count

A

lysis resistant rbc with abnormal hb
nucleated rbc
cryoglobulin
cryofibrinogen
platelet clump
giant platelets
micromegakaryoblasts
megakaryocyte fragements
heparin
monoclonal proteins

75
Q

falsely low wbc

A

leukemia
leukoagglutnation
clotting
smudge cells

76
Q

causes of falsely low neutrophils

A

neutrophil aggregation
neutrophil with hemosiderin granules

77
Q

causes of falsely high lymphocytes

A

nucleated rbc
platelet clumping
giant platelets
malarial parasites
hypolobulated neutrophils

78
Q

causes of falsely high monocytes

A

large reactive lymphocytes
lymphoblast
lymphoma cells
immature granulocytes

79
Q

causes of falsely high eosinophil

A

neutrophil with hemosiderin granules
red cells with malarial pigment

80
Q

what causes a falsely high platelet

A

leukemia
rbc fragments
microorganism
cryoglobulin
cryofibrinogen
hemolysis (in vivo)
hemolysis (in vitro)
microcyte red cell
red cell inclusion
wbc fragments

81
Q

what cause the falsely low platelets

A

platelet clumps
old specimen
partial clotting
giant platelets
platelet satellitism
cold agglutination
clotting
heparin

82
Q

what causes the false high mpv

A

old specimen
determining the MPV too early on EDTA specimen