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HEMATOLOGY 1 > pbs, hematopoiesis > Flashcards

pbs, hematopoiesis Flashcards

(52 cards)

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1
Q

what is the critical value for hematocrit

A

<20%
>65%

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2
Q

what is the critical value for hemoglobin

A

<70 g/L
>200g/L

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3
Q

what is the critical value for RETICULOCYTE

A

> 20

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4
Q

what is the critical value for WBC COUNT

A

<2,000 IF NEW PX OR 1, 000 DIFFERENCE FROM THE PREVIOUS RESULTS IF LESS THAN 4,000

> 50,000 FOR NEW PATIENT

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5
Q

what is the critical value for BLOOD SMEAR

A

NEUTROPHILIC PHAGOCYTOSIS
ABNORMAL LEUKOMOID REACTION
SICKLE CELL
SCHISTOCYTE
BLAST FORMS
PRESENCE OF INTRACELLULAR ORGANISM

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6
Q

what is the critical value for PLATELETS

A

< 20,000/UL AND NOT PREVIOUSLY. REPORTED

> 1,000,000/UL

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7
Q

PROTHOMBIN TIME

A

40 SECONDS

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8
Q

2 sources of specimen for pbs

A

EDTA BLOOD
ANTICOAGULANT FREE BLOOD

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9
Q

WHAT ARE THE ADVANTAGES OF USING EDTA FOR PBS?

A

MULTIPLE BLOOD SMEAR CAN BE MADE
THE BLOOD SMEAR MAY BE PREPARED LATER
EDTA PREEVENTS PLATELET CLUMPING

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10
Q

WHAT ARE THE DISADVANTAGES OF USING EDTA

A

PLATELETS SATELLITOSIS
EDTA INDUCED PLATELET CLUMPING

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11
Q

WHAT ARE THE EFFECTS OF 2 DISADVANTAGES

A

PLATELET SATELLITOSIS: PSEUDOTHROMBOCYTOPENIA

EDTA INDUCED PLATELET CLUMPING: PSEUDOTHROMBOCYTOPENIA AND PSEUDOLEUKOCYTOSIS

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12
Q

WHAT ARE THE REMEDIES FOR PSUEDOTHROMBOOCYTOPENIA AND PSUEDOLEUKOCYTOSIS

A

RECOLLECT BLOOD SPECIMEN THEN USE 3.2% SODIUM CITRATE

THEN AFTER YOU GOT THE RESULT COMPUTE THEM

PLATELET CNT RESULT X 1.1
WBC CNT RESULT X 1.1

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13
Q

THIS KIND OF SPECIMEN COLLECTION IS BEST FOR EVALUATION BLOOD CELL MORPHOLOGY

A

ANTICOAGULANT FREE BLOOD

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14
Q

ADVANTAGES OF ANTICOAGULANT-FREE BLOOD

A

MADE BESIDE THE PX
SOME ARTIFACT CAN BE PREVENTED

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15
Q

MOST FREQUENTLY USED METHOD IN BLOOD FILM PREPARATION

A

TWO-GLASS SLIDE METHOD (MANUAL WEDGE TECHNIQUE)

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16
Q

WHAT ARE THE 2 SLIDES BEING USED

A

PUSHER SLIDE
FILM SIDE

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17
Q

WHAT IS THE ANGLE BETWEEN THESE TWO SLIDES?

A

30-45 DEGREE

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18
Q

IF THE ANGLE OF THE SPREADER IS TOO HIGH WHAT WILL HAPPEN?

A

THICKER SMEAR

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19
Q

IF THE ANGLE OF THE SPREADER IS TOO LOW WHAT WILL HAPPEN?

A

THINNER SMEAR

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20
Q

WHAT IS THE LENGTH OF PBS ?

A

2-3 MM

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21
Q

THE PROXIMITY OF BLOOD TO THE GLASS SLIDE

22
Q

WHAT WILL HAPPEN IF THE SIZE OF THE DROP OF BLOOD IS TOO LARGE?

Study These Flashcards
A

THICKER
LONGER SMEAR

23
Q

WHAT WILL HAPPEN IF THE SIZE OF THE DROP OF THE BLOOD IS TOO SMALL?

Study These Flashcards
A

THINNER
SHORTER SMEAR

24
Q

WHAT WILL HAPPEN IF THE SPEED OF THE SPSREADER IS TOO FAST

Study These Flashcards
A

THICKER SMEAR

25
WHAT WILL HAPPEN IF THE SPEED OF THE SPREADER IS TOO SLOW
THINNER SMEAR AND POOR WBC DISTRIBUTIO
26
WHAT WILL HAPPEN IF THE HCT OF THE PATIENT IS TOO HIGH ?
LOWER THE ANGLE AS LOW AS 25 DEGREE
27
WHAT WILL HAPPEN IF THE HEMATOCRIT OF THE PATIENT IS TOO LOW
ANGLE SHOULD BE RAISED
28
WHAT IS THE PATTER OF LONGITUDINAL (SCANNING METHOD)
TAIL TO HEAD
29
WHAT. IS THE PATTERN OF THE BATTLEMENT ?
BACK AND FORTH SERPENT
30
what is the purpose for blood smear staining
for evaluation of cellular morphology
31
what are the 3 important solutions used for blood smear staning.
fixative stain buffer
32
any stain which contains methylene blue and a halogenated fluorescein dye
romanowsky type stain
33
most commoonly used type of stain in the hematology laboratory
ROMANOWSKY-TYPE STAIN
34
EXAMPLES OF ROMANOWSKY BASED STAINS
WRIGHT STAIN GIEMSA STAIN MAY-GRUNWALD STAIN
35
3 TECHNIQUES OF STAINING
MANUAL AUTOMATED QUICK
36
TECHNIQUE THAT FLOOD THE SLIDE WITH WRIGHT STAIN ALLOW THE STAIN TO REMAIN ON THE SLIDE FOR 1-3 MINS BUFFER IS THEN ADDED
MANUAL TECHNIQUE
37
TECHNIQUE GENERALLY 5-10 MINS TO STAIN A BATCH SLIDES EXAMPLES MIDAS 3 HEMA TEK COUTER LH SYSMEX SP-10
AUTOMATED TECHNIQUE
38
CORRECT COLOR OF PBS MACROSCOPIC
PINK TO PURPLE
39
CORRECT CLOR OF THESE CELLS MICROSCOPICALY RBC
ORANGE TO SALMON PINK
40
CORRECT CLOR OF THESE CELLS MICROSCOPICALY WBC NUCLEI
PURPLE TO BLUE
41
CORRECT CLOR OF THESE CELLS MICROSCOPICALY NEUTROPHIL CYTOPLASMA
PINK TO TAN
42
CORRECT CLOR OF THESE CELLS MICROSCOPICALY EOSINOPHIL GRANULES
BRIGHT ORANGE
43
WHAT ARE THE USUAL CAUSES OF THESE PROBLEMS RBC: GRAY WBC: TOO DARK EOSINOPHIL: GRAY
STAIN/BUFFER TOO BASIC INADEQUATE RINSING HEPARINIZED BLOOD WAS USED
44
WHAT ARE THE USUAL CAUSES OF THESE PROBLEMS RBC: OO PALE OR ARE RED WBC: BARELY VISIBLE
STAIN/BUFFER IS TOO ACIDIC UNDER BUFFERING OVER RINSING
45
BLOOD FILM BLUUER THAN NORMAL WHAT IS THE PROBABLE REASON?
INCREASED BLOOD PROTEINS
46
BLOOD HAS A GRAINY APPEARANCE WHAT COULD BE THE PROBABLE REASOON
RBC AGGLUTINATION
47
HOLES ALL OVER THE FILM WHAT IS THE PROBABLE REASON
INCREASED LIPID LEVEL
48
BLUE SPECKS WHAT COULD BE THE PROBABLE. REASON>
MARKEDLY INCREASED WBC. COUNTS AND PLATELET COUNTS
49
STORAGE OF THE BLOODD SMEAR. SLIDES
AT LEASST 7 DAYS BEFORE PROPER DISPOSAL
50
USED TO EXAMINE THE NULEAR DETAILS OF WBC USDEE ALSO FOR THE TABULATION OF THE ACTUAL WBC DIFFERENTIAL AND ESTIMATION OF PLATELET COUNT
100X OIO
51
USED ALSO TO ESTIMATE TOTAL WBC COUNT
40X HIGH DRY OR 50 X OIO
52
USED TO ASSES OVERALL FIL QUALITY COLOR AND DISTRIBUTION OF CELLS LOCATE RARE ABNORMAL WBC USED TO DETECT SNOWPLOW EFFECT USED TO DETECT FIBRIN STRANDS RECOGNIZE ROULEAUX FROMATION QUICKLY DETECT UNEXPECTED PARASITE ASSESS THE ARE AVAILABLE FOR SUITABLE EXAMINATION
10 X OBJECTIVE

HEMATOLOGY 1 (8 decks)

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