iPSC Flashcards
(11 cards)
1
Q
What is the first stage of embryo
A
oocyte - diploid
2
Q
What is after oocyte?
A
morula - pluripotent
3
Q
After morula?
A
blastula - two distinct cell populations
4
Q
what are the two distinct cell populations in the blastula?
A
inner and outer cell mass
5
Q
What is the final stage?
A
gastrula - endoderm, mesoderm and ectoderm
6
Q
What are the molecules which are secreted by the organs which helps stem cells know their fate?
A
morphogens
7
Q
Types of stem cell
A
embryonic
adult
induced pluripotent
8
Q
How do you make iPSC
A
you use 4 transcription factors - cMic, Klf-4, Oct-3/4 and Sox-3
9
Q
When were the first human iPSC made
A
2007
10
Q
How to culture stem cells
A
culture dishes with culture media at 37 ° and 5% CO
11
Q
A
