KARYOTYPE AND FISH

Question 1

karyotype technique

Answer 1

1. culture
2. harvest
3. analyze

Question 2

1. culture

Answer 2

1. sample collection= blood, bone marrow, cord blood, chorionic villus, skin, tumor, and products of conception
2. culture in growth medium= stimulate mitosis
3. incubate for 3 days = 37-degree celsius for 72 hours

Question 3

Harvest

Answer 3

1. stop mitosis in metaphase= mitotic arrest, spindle arrest

2. add hypotonic solution= KCl, causes the cell to swell and burst, metaphase spread for visualization