L24 Flashcards
1
Q
is Pfu polymerase processive
A
yes
2
Q
does Pfu have exonuclease (3’ to 5’) activity
A
yes - lower error rate because of it
3
Q
is the GC 3’ end clamp wanted by primers
A
yes
4
Q
primers are designed to
A
span genome regions that contain repeated sequences
5
Q
how many sets in codis
A
13
6
Q
what tests disease
A
PCR test
7
Q
mutagenesis
A
primer developed with a single nucleotide mismatch or a small insertion/deletion off a plasmid
8
Q
does sequence assembly require a template?
A
no - the primers are templates for eachother
9
Q
Bliss
A
blacklist sequence screening
