Lab 1 - Haematology past questions Flashcards Preview

Physiology 1 > Lab 1 - Haematology past questions > Flashcards

Flashcards in Lab 1 - Haematology past questions Deck (15)
Loading flashcards...
1
Q

What are the effects of heparin?

A

It prevents blood from coagulating by catalysing the thrombin-antithrombin reaction

2
Q

What is the function of methanol in fixation?

A

Fix the smear and precipitate the proteins

3
Q

Buffy coat, explain

A

White blood cells have lower density than red, and will form thin layer on the top after centrifugation

4
Q

How do you obtain blood serum

A

Centrifuge blood sample where fibrinogen is changed into fibrin by shaking in flask containing small glass beads

5
Q

How do you get Defirbrinated blood?

A

Shake flask with blood and small glass beads to change fibrinogen into fibrin

6
Q

What happens with the hematocrit level while increasing the epinephrine level?

A

It will increase

7
Q

Give examples of anticoagulant agents.

A

Sodium Citrate

EDTA: Ethylene Diamine Tetracetic Acid

8
Q

How do you perform the Lee white method?

A

Tilt blood in tube in intervals in 3 minutes to see if it coagulates

9
Q

What is the Definition of hematocrit

A

Ratio of red blood cells to the total blood volume

10
Q

Venous hematocrit is larger than arterial, explain why?

A

Because of ion transport and water flux associated with the CO2 transport

11
Q

How does Increased erythrocyte sedimentation rate happen?

A

By displacing the albumin, the surface charge decrease

12
Q

Describe the neutrophil granulocytes (Mammals )

A

Nucleus:

  • Beanshape (young),
  • Elongated (middleage)
  • Segmented (mature),

Cytoplasm:
- Light pink cytoplasm,

Granules:

  • Different granules
  • 10 μm diameter

Enzymes:

  • Lysozyme
  • Myeloperoxidase
13
Q

Describe the hetrophil granulocytes (birds)

A

Nucleus:
- Seveeral segments

Cytoplasm:
- Pink cytoplasm

Granules:
- Pale red granules)

14
Q

Describe the eosinophil granulocytes

A

Nucleus:
- Segmented (usually bi-lobed)

Granules:

  • Large bright red granules
  • 14 μm diameter

Enzmes:

  • Histaminase,
  • Acid & alkaine phosphatase
  • Eosinphil basic & cationic protein
15
Q

Describe the basophil granulocytes

A

Nucleus:
- Hardly segmented

Granules

  • Large blue granules,
  • 15 μm diameter

Enzymes:
- Protease