Lab 5 Flashcards

(4 cards)

1
Q

What is the purpose of the dye in gel electrophoresis?

A

1) Provides density so you can see that you are loading in gel correctly
2) Moves more quickly through gel than DNA so you can know when to stop the voltage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What does E Value represent in a BLAST?

A

The chance that the match is by chance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What does BLAST do?

A

Compares 16s RNA base sequence of unknown bacteria against a database of known base sequences

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What can you tell by the gel electropheresis?

A

The size of the fragment of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly