Lecture 8

Question 1

Bacteriphage as potential therapy

Answer 1

When viruses were discovered this was super popular but quickly died out

Question 2

Penecillin

Answer 2

replaces viruses as the primary therapy against infections

Question 3

Cryo-EM

Answer 3

allows detailed 3D reconstruction of phage particles (phage T7)

Question 4

MOI

Answer 4

multiplicity of infection a low proportion of cells become infected with virus

Question 5

latent period

Answer 5

time when virus is mutliplying in the cell

Question 6

Burst Size

Answer 6

Ratio of input to output bacteriophage (100 or more)

Question 7

What does a plaque contain on a lawn?

Answer 7

contains progeny of single infecting virus (a single lineage)

Question 8

Temperate phage

Answer 8

can go through lytic cycle or lysogenic cycle

Question 9

What color plaque do lytic produce versus temperate phage?

Answer 9

Lytic-clear

Temperate- turbid plaques

Question 10

What is a maltose porin (LamB)?

Answer 10

a porin created in cell membrane of E.coli when grown in maltose

Question 11

what decides wheather the lamba will enter the lytic or lysogenic cyce?

Answer 11

cll and cro

Question 12

When cll predominates what happens?

Answer 12

Lysogen formed

Question 13

When cro predominates:

Answer 13

Lambda enters lytic cycle

Question 14

What does stress do?

Answer 14

induces lytic cycle through SOS response (actiates RecA which inactivates LexA repressor)

Question 15

How does lamba inssert into chromosome?

Answer 15

Site specific recombination