Lab 8 - Blood sugar Flashcards Preview

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Flashcards in Lab 8 - Blood sugar Deck (14):
1

What is the blood sugar?

The conc. of all monosaccharides present in the blood.

2

Blood sugar level, with exceptans

In mammalian: 4-5 mmol/l, except Ru: 2-3 mmol/l. Avian: 8-9 mmol/l

3

Which organs require large quantity of sugar for energy supply?

The brain and kidney

4

Ways to maintain stabile glucose level

1.Abs. from intestines
2.Glycogenolysis (phosphorolysis) in liver, prod. glucose from glycogen.
3.Gluconeogenesis in liver

5

Which hormones influence the blood sugar conc. and how?

1.Insulin - decr.
2.Glucagon - incr.
3.Adrenalin - incr.
4.Glucocorticoids (pl.cortisol) - incr.
5.Growth hormone (GH/STH) - incr.
6.ACTH - Incr.

6

What are the place of prod. for...
1.Insulin
2.Glucagon
3.Adrenalin
4.Glucocorticoids (pl.cortisol)
5.Growth hormone (GH/STH)
6.ACTH

1. Pancreas beta-cells
2.Pancreas alpha-cells
3.Adrenal medulla
4.Adrenal cortex (zona fasciculata)
5.Adenohypophysis
6.Adenohypophysis

7

What increases the...
1.Insulin
2.Glucagon
3.Adrenalin
4.Glucocorticoids (pl.cortisol)
5.Growth hormone (GH/STH)
6.ACTH

1.GG in the liver, GL on the periphery glucose uptake by the cells (requires GLUT-4 transporter protein)
2.GGL in liver, GNG in liver
3.GGL in muscles
4.GNG (prim. from a.a.)
5.Lipolysis
6.Glucocorticoid prod. of adrenal cortex

8

What decreases the...
1.Insulin
2.Glucagon
3.Adrenalin
4.Glucocorticoids (pl.cortisol)
5.Growth hormone (GH/STH)
6.ACTH

1.GGL in liver an muscles, LL, GNG in liver
2.GG in liver, GL in liver
3.GG in muscles
4.GL
5.GNG (from a.a.)
6. -

9

The lab-tests

1.Effect of insulin, adrenalin and glucose injection on blood sugar conc. of rabbit
2.Determination of blood glucose conc. by GOD-POD method

10

Materials in "1.Effect of insulin, adrenalin and glucose injection on blood sugar conc. of rabbit"

-Rabbit
-Insulin injection (actrapid inj.)
-Adrenalin inj. (tonogen inj.)
-Sterile glucose sol. (0.3mol/l)
-70% ethanol
-Eppendorf tubes treated with heparin
-Needle for drawing of blood (18-19, 23G)
-Syringes, scissors, cotton wool

11

Which method is used to estimate blood glucose conc.?

-GOD-POD - Glucose oxidase peroxidase
-Glucometer: enzymes in test strip induce a color change or el.charge. This prod. is translated into a glucose measurment.

12

"2.Determination of blood glucose conc. by GOD-POD method":
1.Which form are the enzymes available in?
2.The reaction?
3. How can it be measured?
4.Observations?

1.Lypophilized forms
2.Glucose prod. gluconic acid by GOD with water uptake and H2O2 prod. POD tranform the H2O2 to H2O in Trinder´s indicator R.
3.546 nm spectrophotometry
4.Colourful condensate

13

What is in Trinder´s indicator reaction?

4-aminoantipyrine and phenol reagent

14

Materials in "2.Determination of blood glucose conc. by GOD-POD method"

-frehsly collected blood inhibited in clotting
-TCA (10%)
-eppendorf tubes
-Colour reagent: GOD and POD dissolved in phosphate buffer and colour-forming agent (4-aminoantipryin and phenol)
-Spectrohptometry
-Micropipette
-37% water bath