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Microbiology 210 > Lab Exam > Flashcards

Flashcards in Lab Exam Deck (55)
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1

Stage

Fixed platform with an opening in the center allowing for passage of light from illuminating source to lens system

2

Mechanical stage

Can be moved vertically or horizontally by means of adjustment controls.

3

Illumination

Light source positioned in the base of the instrument

4

condenser

Component directly under the stage contains two sets of lenses that collect and concentrate light

5

Iris diaphragm

Shutter controlled by a lever that is used to regulate the amount of light entering the lens system

6

Body tube

Above the stage, attached to the arm of th microscope'

7

Ocular lens

Eyepiece lens

8

Nosepiece

Moveable, contains objective lens

9

Coarse adjustment and fine adjustment knobs

Below stage

10

Magnification

Enlargement of a specimen using a two lens system: ocular and objective

11

Objective produces what kind of image?

Real image

12

Ocular lens produces the

Final image

13

Resolving power

How far apart two adjacent objects must be before a given lens shows them as discrete entities. When two objects look as one, the resolution has been lost

14

Numerical aperture

Function of the diameter of the objective lens in relation to its focal length. RP=wavelength of light/2x numerical aperture

15

refractive index

The bending power of light passing through air from the glass slide to the objective lens

16

As the magnification of the lens increases,

The working distance decreases and the numerical aperture of the objective lens increases

17

Parfocal

When one lens is in focus, others will also have the same focal length

18

Heat fixation

Rapid movement of specimen over flame fixing to the slide

19

Sub culturing

Transfer of organism from one medium to another

20

Pure culture

Containing only one specimen

21

Uniform fine turbidity

Finely dispersed growth throughout

22

Flocculent

Flakey aggregates dispersed throughout

23

Pellicle

Thick pad like growth on surface

24

Sediment

Concentration of growth at the bottom of broth culture may granular, flaky or flocculent

25

Crateriform

Liquefied surface is saucer-shaped

26

Stratiform

Complete liquefaction of the upper half of the medium

27

Simple staining

Stained with single reagent: methylene blue, crystal violet and carbon fuchsin

28

Diplo

Pair

29

Strepto

Chain

30

Staphlyo

Cluster