[LAB] Lab Safety and Microscopy

Question 1

How microorganisms are transmitted

Answer 1

chain of infection

Question 2

• Instituted by CDC in 1987
• All patients are considered to be possible carriers of bloodborne pathogens

Answer 2

Universal Precaution

Question 3

• Not limited to bloodborne pathogens
• Consider ALL body fluids and most body substances to be potentially infectious
• Do not recommend handwashing after removing gloves unless visual contamination is present

Answer 3

Body Substance Isolation

Question 4

Who made the standard precautions?

Answer 4

Center for Disease and Control (CDC)
Healthcare Infection Control Practices Advisory Committee (HICPAC)

Question 5

• combined UP and BSI guidelines in 1996 by CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC)

Answer 5

Standard Precautions

Question 6

A law monitored and enforced by OSHA that indicates: any accidental exposure to a possible bloodborne pathogen must be immediately reported to a supervisor.

Answer 6

Occupational Exposure to Bloodborne Pathogens Standard

Question 7

What is the primary mode of transmission?

Answer 7

hand contact

Question 8

dilution of sodium hypochlorite for disinfecting the sink

Answer 8

1:5 or 1:10

Question 9

When chemical spills came in contact with your eye and skin, how long will you wash your eye or skin with water?

Answer 9

15 minutes

Question 10

True or False.

Acid should always be added to water

Answer 10

True

Question 11

Who made the symbol for 4 hazards (health, fire, specific, and reactivity)?

Answer 11

National Fire Protection Association

Question 12

A document that provides workers with procedures for safety handling or working with a particular substance.

Answer 12

Material Safety Data Sheet

Question 13

Enumerate the meaning of RACE.

Answer 13

Rescue
Alarm
Contain
Extinguish

Question 14

An instrument that magnifies objects that are too small to be seen by the naked eye, producing an image in which the objects appear larger.

Answer 14

Microscope

Question 15

Identify the part of the microscope.

• carries the optical parts in the upper part of the microscope

Answer 15

head

Question 16

Identify the part of the microscope.

• Acts as support
• Also carries the microscopic illuminators

Answer 16

base

Question 17

Identify the part of the microscope.

• Connects the base and the head of the microscope
• Support to the head of the microscope

Answer 17

arm

Question 18

Identify the part of the microscope.

• Ocular
• Part used to look through the microscope
• Found at the top of the microscope
• Standard magnification is 10x

Answer 18

eyepiece

Question 19

What is the standard magnification?

Answer 19

10x

Question 20

Identify the part of the microscope.

• Major lenses used for specimen visualization
• Has a magnification power of 4x,10x, 40x, and 100x (scanner, LPO, HPO, and OIO ̧ respectively)

Answer 20

objective lenses

Question 21

Identify the part of the microscope.

• Revolving turret
• Holds the objective lenses

Answer 21

nosepiece

Question 22

Identify the part of the microscope.

• Hole in the microscopic stage
• The light is transmitted from the source to the stage

Answer 22

aperture

Question 23

Identify the part of the microscope.

• Light source
• Used instead of a mirror

Answer 23

microscopic illuminator

Question 24

Identify the part of the microscope.

• Used to collect and focus light
• Ensures clear, and sharp images with high magnification

Answer 24

condenser

Question 25

Identify the part of the microscope. - controls the amount of light that reaches the specimen

Answer 25

diaphragm

Question 26

Identify the type of microscope. - Frequently used in the clinical laboratory - Objects appear dark on a light background - Used in routine urinalysis

Answer 26

bright-field

Question 27

Identify the type of microscope. - Enhances visualization of elements with low refractive indices - Used for gaining contrast in a translucent specimen without staining

Answer 27

phase-contrast

Question 28

Identify the type of microscope. - Use of polarized light aids in the identification of crystals, oval fat bodies, fatty casts, and crystals - Elements seen under polarized light are birefringent

Answer 28

polarizing

Question 29

Identify the type of microscope. - Aids in the identification of Treponema pallidum - To enhance visualization of specimens that cannot be seen easily viewed with a bright-field microscope - Often used for unstained specimens - Specimen appears light against the black background

Answer 29

dark-field

Question 30

Identify the type of microscope. - Used to detect bacteria and viruses within cells and tissues - Allows visualization of naturally fluorescent microorganisms or those stained by a fluorescence dye including labeled antigens and antibodies

Answer 30

fluorescence

Question 31

Identify the type of microscope. - Produces a three-dimensional microscopy image and layer-by-layer imaging of a specimen

Answer 31

interference-contrast