LAB M2 Ex2-3

Question 1

other name for rocking microtome

Answer 1

cambridge

Question 2

rocking microtome inventor

Answer 2

Paldwell Trefall 1881

Question 3

knife profile of rocking microtome

Answer 3

plano concave

Question 4

rocking microtome is used for

Answer 4

celloidin embedded blocks

large paraffin emebedded blocks

Question 5

thickness of rocking microtome tissue

Answer 5

10-12 um

Question 6

other term for rotary microtome

Answer 6

minot

Question 7

knife profile of rotary microtome

Answer 7

biconcave

planoconcave

Question 8

rotary microtome is used for

Answer 8

paraffin or resin embedded blocks

Question 9

most common microtome for routine lab

Answer 9

rotary/ minot microtome

Question 10

thickness of rotary microtome sections

Answer 10

3- 5 um

Question 11

two types of sliding microtome

Answer 11

base sledge

standard sliding

Question 12

knife is stationary and block is manipulated

Answer 12

base sledge

Question 13

block is stationary and knife is manipukated

Answer 13

standard sliding

Question 14

knife of bade sledge

Answer 14

plano concave

plane wedge knife

Question 15

base sledge is used for

Answer 15

large blocks (whole brain), hard tossues, whole mounts

Question 16

knife of standard sliding microtome

Answer 16

planoconcave

plane wedge

Question 17

use of standard sliding microtome

Answer 17

celloidin – oblique knife

large refractory paraffin - straight knife

Question 18

inventor of sliding microtome

Answer 18

adams 1789

Question 19

inventor of freezing microtome

Answer 19

Queckett 1848

Question 20

knife of freezing microtome

Answer 20

plane wedge

Question 21

use of freezing microtome

Answer 21

rapid diagnosis, enzyme, lipids, neurological structures

Question 22

temperature of cryostat

Answer 22

-5 to -30 degrees

Question 23

size very thin sections of ultrathin microtome

Answer 23

60-100 nm

Question 24

size of semi thin sections of uktrathin microscope

Answer 24

0.5- 1 um

Question 25

use of ultrathin microtome

Answer 25

electron microscopy

Question 26

advances the block holder towards pr away the knife

Answer 26

coarse handwheel

Question 27

advanves the block only towards the knife by microns

Answer 27

fine advancement wheel

Question 28

prevengs block holder from coming down towards blade

Answer 28

locking mechanisms

Question 29

hokds the blade

Answer 29

blade clamp

Question 30

adjusts the knife angle

Answer 30

knife tilt

Question 31

guides ribbins away from the blade towards the operator

Answer 31

face plate

Question 32

predetermined thickness for sectioning

Answer 32

5-10 um

Question 33

msot common type of microtome knife

Answer 33

steel knives

Question 34

disposable knives are coated in

Answer 34

PTFE polytetrafluoroethylene

Question 35

sharper than diamon knoves but more fragile

Answer 35

glass knives

Question 36

sharper than diamon knoves but more fragile

Answer 36

glass knives

Question 37

very expensive knife with long usage span

Answer 37

diamond knife

Question 38

concave on both sides

Answer 38

biconcave knife

Question 39

length of biconcave knife

Answer 39

120 mm

Question 40

one flat side, one concave side

Answer 40

planoconcave

Question 41

length of planoconcave knife

Answer 41

25 mm

Question 42

has deep curring edge and both straight sides

Answer 42

plane wedge knife

Question 43

length of plane wedge knife

Answer 43

100 mm

Question 44

bevel angle

Answer 44

27- 32

Question 45

cutting angle

Answer 45

15

Question 46

clearance angle for paraffin embedded tissues

Answer 46

2-4

Question 47

clearance angke for frozen sections

Answer 47

5-7

Question 48

angle between the cutring edges

Answer 48

bevel angle

Question 49

angle at which maximum penetrayion at minimum distortion is achieved

Answer 49

cutting angle

Question 50

angle between upper knife and perpendicular line from block

Answer 50

rake angle

Question 51

rake angle for sof tissues

Answer 51

high rake angle

Question 52

rake angle for hard tissues

Answer 52

low rake angle

Question 53

tip of blade scratched the block

Answer 53

too wide clearance angle

Question 54

body of blade scrapes the block

Answer 54

too small clearance angle

Question 55

angle between bl9ck surface and lower level of knife

Answer 55

clearance angle

Question 56

rougher side of belgian yellow for coarse honing

Answer 56

black surface

Question 57

smoother side of belgian yellow

Answer 57

yellow side

Question 58

honing us repeated how many times

Answer 58

20-30

Question 59

applied before stroping

Answer 59

vegetable oil

Question 60

stropping is repeated how many times

Answer 60

40-120