Lab Practical 2

Question 1

Simple stain is for seeing

Answer 1

Morphology

Question 2

Gram stain is

Answer 2

To differentiate gram negative and gram positive bacteria

Question 3

Gram positive bacteria is

Answer 3

Streptococcus
Staphylococcus
Listeria
Corynebacterium
Bacillus
Clostridium

Question 4

Gram Negative Bacteria is

Answer 4

Escherichia
Hemophilus
Neisseria
Salmonella

Question 5

What is the cell wall structure of Gram-Positive bacteria

Answer 5

2 Layers
Thick Peptidoglycan
Plasma Membrane

Question 6

What is the cell wall structure of gram-negative bacteria

Answer 6

3 Layers
Outer Membrane
Thin peptidoglycan
Plasma Membrane

Question 7

Bacillus Anthracis is

Answer 7

Gram Positive
Rod Morphology
Arrangement: Streptobacillus

Causes anthrax

Question 8

Bacillus Cereus is

Answer 8

Gram Positive
Rod Morphology
Arrangement: Streptobacillus
Causes Food poisoning (associated with undercooked rice)
Has Endospores

Question 9

Bordetella Pertussis is

Answer 9

Gram negative
Morphology: Rods, coccobacillus
Arrangement: Single or in pairs
Causes Pertussis or whooping cough

Question 10

Borrelia Burgdoferi is

Answer 10

Gram negative
Morphology: Spirochete
Arrangement: Grouping
Causes Lyme Disease

Question 11

Clostridium Botulinum is

Answer 11

Gram positive
Morphology: Rods
Arrangement: Grouping
Causes Botulism (paralysis), has endospores

Question 12

Clostridium difficile is

Answer 12

Gram positive
Morphology: Rods
Arrangement: Grouping
Normal flora that is nosocomial (hospital borne)

Question 13

Clostridium perfirgens is

Answer 13

Gram Positive
Morphology: rods
Arrangement: grouping
Causes gas gangrene, food poisoning

Question 14

Clostridum tetani is

Answer 14

Gram Positive
Morphology: Rods
Arrangement: Grouping
Causes tetanus

Question 15

Corynebacterium diphtheriae is

Answer 15

Gram Positive
Morphology: Rods
Arrangement: V shape
Causes Diphtheria (an acute, highly contagious bacterial disease causing inflammation of the mucous membranes, formation of a false membrane in the throat that hinders breathing and swallowing, and potentially fatal heart and nerve damage by a bacterial toxin in the blood. It is now rare in developed countries because of immunization.)

Question 16

Escherichia Coli is

Answer 16

Gram negative
Rod Morphology
Arrangement: Grouping
Causes Food poisoning (is food handled comes from poor hygiene after using bathroom)
O157:H7 is the deadly strain.

Question 17

Hemophilus influenzae is

Answer 17

Gram Negative
Rod morphology
Causes Pinkeye, upper respiratory infections

Question 18

Klebsiella pneumoniae is

Answer 18

Gram Negative
Rod Morphology
Causes pneumonia

Question 19

Lactobacillus acidophilus is

Answer 19

Gram Positive
Rod morphology
Normal vaginal flora, maintains acidic vaginal pH

Question 20

Mycobacterium leprae is

Answer 20

Acid-Fast (red)
Morphology - Rods
Disease: Leprosy (Hansen’s disease)

Question 21

Mycobacterium tuberculosis is

Answer 21

Acid-Fast (red)
Morphology- Rods
Disease - Tuberculosis

Question 22

Neisseria gonorrhoeae is

Answer 22

Gram negative
Morphology - Coccus
Arrangement - Diplococci
Causes gonorrhea

Question 23

Neisseria meningitidis is

Answer 23

Gram Negative
Morphology - Cocci
Arrangement - Diplococci
Causes meningitis

Question 24

Pseudomonas aeruginosa is

Answer 24

Gram negative
Morphology - Rods
Nosocomial opportunistic

Question 25

Salmonella typhi is

Answer 25

Gram Negative Morphology - Rods Causes Typhoid fever, spread through contaminated food or water

Question 25

Staphylococcus aureus is

Answer 25

Gram positive Morphology - Coccus Arrangement - Staphylococcus Nosocomial opportunistic, lives in the nose

Question 25

Streptococcus pyogenes is

Answer 25

Gram positive Morphology - coccus Grouping - Streptococcus Causes strep throat Opportunistic, nosocomial

Question 26

Streptococcus pneumoniae is

Answer 26

Gram Positive Morphology - Coccus Arrangement - Diplococci Causes pneumonia, meningitis

Question 27

Treponema pallidum is

Answer 27

Gram negative Morphology - Spirochete Causes Syphilis can be transferred via head of sperm (observable by examining a sperm infected by Treponema pallidum)

Question 28

Organic compounds comprised of carbon and hydrogen atoms are called

Answer 28

hydrocarbons

Question 29

The use of microbes to cleanup pollutants is called

Answer 29

Bioremediation

Question 30

What does ELISA stand for?

Answer 30

Enzyme linked immunosorbent Assay

Question 31

What type of ELISA test did we do in class?

Answer 31

Indirect ELISA

Question 32

What disease did our simulated ELISA test, test for?

Answer 32

HIV

Question 33

What tool did we use in ELISA lab to collect the specimens?

Answer 33

microtiter plate

Question 34

What does the ELISA test look for?

Answer 34

The presence/absence of antibodies against a particular pathogen

Question 35

What transfers the antibodies to the microtiter plate?

Answer 35

Pipets

Question 36

What letter of the alphabet is an antibody shaped like?

Answer 36

Y

Question 37

An antigen binds to what part of the antibody?

Answer 37

The ends of the antibody

Question 38

What organisms were used for bioremediation lab?

Answer 38

Pseudomonas and Penicillium

Question 39

What is the primary stain and the counterstain for endospores?

Answer 39

Malachite Green - Primary Safarin - Counterstain

Question 40

What is the primary stain and the counterstain for acid-fast?

Answer 40

Carbol Fuschin - Primary (red) Methylene blue - Counterstain (blue)

Question 41

What were the two organisms used in the acid-fast staining? which one retained the acid-fast stain?

Answer 41

Staphylococcus epidermidis Mycobacterium non-chromogenium (retained the stain)

Question 42

What were the two organisms used in the endospore staining? Which one retained the endospore stain?

Answer 42

Bacillus cereus (retained the endospore stain) Escherichia coli

Question 43

What shape is spiral without movement? | t

Answer 43

Spirillum

Question 44

What shape is spiral with movement?

Answer 44

Spirochete