lab techniques Flashcards
(7 cards)
1
Q
what are immune mediators
A
- antigens
- antibodies
- cytokines
- cells
2
Q
what does the concentration of immune mediators tell us
A
information of the disease state - mechanism, diagnosis, response to treatment
3
Q
what occurs with a blood group assay
A
- dilute blood sample
- add antibodies against erythrocyte antigens (A, B ,Rh)
- agglutination occurs to determine blood group
4
Q
what does a radioimmunoassay do
A
- competitive antibody binding assay
- cold insulin competes with hot insulin
- higher 125 I insulin means less normal insulin and type 1 diabetes
5
Q
what is the disadvantage of radioimmunoassay
A
radiolabel is hazardous
6
Q
what is the advantage of radioimmunoassay
A
faster than chromatography based methods
7
Q
A