lab techniques Flashcards

(7 cards)

1
Q

what are immune mediators

A
  • antigens
  • antibodies
  • cytokines
  • cells
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2
Q

what does the concentration of immune mediators tell us

A

information of the disease state - mechanism, diagnosis, response to treatment

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3
Q

what occurs with a blood group assay

A
  • dilute blood sample
  • add antibodies against erythrocyte antigens (A, B ,Rh)
  • agglutination occurs to determine blood group
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4
Q

what does a radioimmunoassay do

A
  • competitive antibody binding assay
  • cold insulin competes with hot insulin
  • higher 125 I insulin means less normal insulin and type 1 diabetes
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5
Q

what is the disadvantage of radioimmunoassay

A

radiolabel is hazardous

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6
Q

what is the advantage of radioimmunoassay

A

faster than chromatography based methods

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7
Q
A
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