Lab Techniques Flashcards
(24 cards)
Suggest two precautions which should be put in place when working with chemicals
Ensure pregnant workers do not use it Wear gloves Wear a lab coat Have an established procedure for spills Maintain a clean working area before and after use Ensure they are disposed of safely
What is PPE
personal protective equipment
Why is PPE used
To protect the body from harm
Give an example of PPE which is appropriate for working with UV light
A lab coat
Safety glasses
A face shield
Why are buffers used in an experiment
The keep the ph constant
Name a piece of equipment used to measure the absorbance of a solution
Colorimeter
What type of cells produce antibodies
B lymphocytes
What term is used to describe fused cells
Hybridomas
Name the chemical used to fuse cells together
PEG polyethylene glycol
Other than a microscope, what piece of equipment is required to perform a cell count
Haemocytometer
Name a biological control measure
Use a more sustainable strain of micro organism
i.e. Less virulent
Introducing genes to the organisms which prevent it from surviving outside the lab
What are autopipettes used for
Measuring small volumes precisely
Explain how autopippettes are used
Like a syringe but have two stops
1st for drawing
2nd for dispensing
What are curettes used for
To dispense precise volumes of liquid reagents i.e. For titration
What is a dilution series
Repeated dilution from a stock solution
How is a dilution series performed
1cm3 stock solution - tube + 9cm3 = 10-1 - 1cm3 10-1 + 9 cm3 of water = 10-2
Describe one use of a dilution series
If there are too many cells to count
What is a colorimeter
A device used to measure the absorbance of a specific wavelength of light by a solution
How is a colorimeter used
It works by passing a light beam, at a specific wavelength, through a cuvette containing a sample solution
What is a buffer
A solution whose ph changes very little when a small volume of strong acid or base is added to it
Give an example of a buffer found in the human body
Carbonic acid to maintain the ph of blood
What name is given to the test which allows the concentration of a protein in a solution to be determined
Bradford protein assay
Bradford reagent
Explain how this test is performed
Use a known concentration to plot a standard curve to find an unknown concentration
What is a centrifuge
A piece of equipment that spins an object at high speed
