Lab Techniques

Question 1

Suggest two precautions which should be put in place when working with chemicals

Answer 1

Ensure pregnant workers do not use it
Wear gloves
Wear a lab coat
Have an established procedure for spills
Maintain a clean working area before and after use
Ensure they are disposed of safely

Question 2

What is PPE

Answer 2

personal protective equipment

Question 3

Why is PPE used

Answer 3

To protect the body from harm

Question 4

Give an example of PPE which is appropriate for working with UV light

Answer 4

A lab coat
Safety glasses
A face shield

Question 5

Why are buffers used in an experiment

Answer 5

The keep the ph constant

Question 6

Name a piece of equipment used to measure the absorbance of a solution

Answer 6

Colorimeter

Question 7

What type of cells produce antibodies

Answer 7

B lymphocytes

Question 8

What term is used to describe fused cells

Answer 8

Hybridomas

Question 9

Name the chemical used to fuse cells together

Answer 9

PEG polyethylene glycol

Question 10

Other than a microscope, what piece of equipment is required to perform a cell count

Answer 10

Haemocytometer

Question 11

Name a biological control measure

Answer 11

Use a more sustainable strain of micro organism
i.e. Less virulent

Introducing genes to the organisms which prevent it from surviving outside the lab

Question 12

What are autopipettes used for

Answer 12

Measuring small volumes precisely

Question 13

Explain how autopippettes are used

Answer 13

Like a syringe but have two stops
1st for drawing
2nd for dispensing

Question 14

What are curettes used for

Answer 14

To dispense precise volumes of liquid reagents i.e. For titration

Question 15

What is a dilution series

Answer 15

Repeated dilution from a stock solution

Question 16

How is a dilution series performed

Answer 16

1cm3 stock solution - tube + 9cm3 = 10-1 - 1cm3 10-1 + 9 cm3 of water = 10-2

Question 17

Describe one use of a dilution series

Answer 17

If there are too many cells to count

Question 18

What is a colorimeter

Answer 18

A device used to measure the absorbance of a specific wavelength of light by a solution

Question 19

How is a colorimeter used

Answer 19

It works by passing a light beam, at a specific wavelength, through a cuvette containing a sample solution

Question 20

What is a buffer

Answer 20

A solution whose ph changes very little when a small volume of strong acid or base is added to it

Question 21

Give an example of a buffer found in the human body

Answer 21

Carbonic acid to maintain the ph of blood

Question 22

What name is given to the test which allows the concentration of a protein in a solution to be determined

Answer 22

Bradford protein assay

Bradford reagent

Question 23

Explain how this test is performed

Answer 23

Use a known concentration to plot a standard curve to find an unknown concentration

Question 24

What is a centrifuge

Answer 24

A piece of equipment that spins an object at high speed