(Laboratory) Antimicrobial Susceptibility Testing Flashcards by SHEKINAH MOLO

List down the step-by-step procedure in AST:

Prepare your pure inoculum using sterile distilled water/NSS. Add 3-5 colonies from your source plate and add it into the tube containing sterile distilled water/NSS. Match its turbidity with the McFarland standard.
Dip a sterile swab into the pure inoculum. Press the swab into the sides of the tube to remove excess fluid.
Inoculate the MHA using the lawn culture method. Using the swab, make a cross across your MHA plate. Then inoculate it from 3 directions to make a lawn. Pass the swab around the edge of the agar surface 3 times.
After 5 minutes, place the antibiotic discs along the surface of the plate using sterile forceps. Make sure the discs are spaced evenly, with approximate distance of 15 mm from the edge of the plate. Gently press the discs on the agar to ensure contact.
Within 30 minutes of preparation, incubate the plates overnight.
Measure the zone of inhibition using a vernier caliper or ruler.
Interpret the zone sizes using the chart.

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Gram-negative Antibiotics:

Chloramphenicol
Gentamicin
Cephalotin
Ampicillin
Cefuroxime
Carbenicillin
Ticarcillin
Tetracycline
Kanamycin
Amoxicillin
Ciprofloxacin
Nitrofurantoin
Ertapenem
Ampicillin-Sulbactam

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Gram-positive Antibiotics:

Erythromycin
Amoxicillin/Clavulanic Acid
Penicillin G
Streptomycin
Novobiocin
Oxacillin
Cephalotin
Aztreonam
Nalidixic Acid
Vancomycin
Ciprofloxacin
Clindamycin
Cefoxitin
Bacitracin

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Abbreviation of Chloramphenicol antibiotic:

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Abbreviation of Gentamicin antibiotic:

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Abbreviation of Cephalotin antibiotic:

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Abbreviation of Ampicillin antibiotic:

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Abbreviation of Cefuroxime antibiotic:

CXM

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Abbreviation of Carbenicillin antibiotic:

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Abbreviation of Ticarcillin antibiotic:

TiC

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Abbreviation of Tetracycline antibiotic:

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Abbreviation of Kanamycin antibiotic:

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Abbreviation of Amoxicillin antibiotic:

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Abbreviation of Ciprofloxacin antibiotic:

CIP

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Abbreviation of Nitrofurantoin antibiotic:

NI/F

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Abbreviation of Ertapenem antibiotic:

ETP

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Abbreviation of Ampicillin-Sulbactam antibiotic:

SAM

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Abbreviation of Erythromycin antibiotic:

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Abbreviation of Amoxicillin/Clavulanic Acid antibiotic:

AmC

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Abbreviation of Penicillin G antibiotic:

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Abbreviation of Streptomycin antibiotic:

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Abbreviation of Novobiocin antibiotic:

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Abbreviation of Oxacillin antibiotic:

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Abbreviation of Cephalotin antibiotic:

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Abbreviation of Aztreonam antibiotic:

ATM

Abbreviation of Nalidixic Acid antibiotic:

Abbreviation of Vancomycin antibiotic:

Abbreviation of Ciprofloxacin antibiotic:

CIP

Abbreviation of Clindamycin antibiotic:

Abbreviation of Cefoxitin antibiotic:

FOX

Abbreviation of Bacitracin antibiotic:

Resistance value of Chloramphenicol:

≤12

Resistance value of Gentamicin:

≤13

Resistance value of Cephalotin:

≤14

Resistance value of Ampicillin:

≤13

Resistance value of Cefuroxime:

≤14

Resistance value of Carbenicillin:

≤19

Resistance value of Ticarcillin:

≤14

Resistance value of Tetracycline:

≤14

Resistance value of Kanamycin:

≤13

Resistance value of Amoxicillin:

≤19

Resistance value of Ciprofloxacin:

≤13

Resistance value of Nitrofurantoin:

≤14

Resistance value of Ertapenem:

≤12

Resistance value of Ampicillin-Sulbactam:

≤11

Intermediate value of Chloramphenicol:

13-17

Intermediate value of Gentamicin:

14-17

Intermediate value of Cephalotin:

15-17

Intermediate value of Ampicillin:

14-16

Intermediate value of Cefuroxime:

15-17

Intermediate value of Carbenicillin:

20-22

Intermediate value of Ticarcillin:

15-19

Intermediate value of Tetracycline:

15-18

Intermediate value of Kanamycin:

14-17

Intermediate value of Amoxicillin:

No value

Intermediate value of Ciprofloxacin:

14-17

Intermediate value of Nitrofurantoin:

15-16

Intermediate value of Ertapenem:

13-15

Intermediate value of Ampicillin-Sulbactam:

12-14

Susceptible value of Chlorampenicol:

≥18

Susceptible value of Gentamicin:

≥18

Susceptible value of Cephalotin:

≥18

Susceptible value of Ampicillin:

≥17

Susceptible value of Cefuroxime:

≥18

Susceptible value of Carbenicillin:

≥23

Susceptible value of Ticarcillin:

≥20

Susceptible value of Tetracycline:

≥19

Susceptible value of Kanamycin:

≥18

Susceptible value of Amoxicillin:

≥20

Susceptible value of Ciprofloxacin:

≥18

Susceptible value of Nitrofurantoin:

≥17

Susceptible value of Ertapenem:

≥16

Susceptible value of Ampicillin-Sulbactam:

≥15

Resistant value of Erythromycin:

≤13

Resistant value of Amoxicillin/Clavulanic Acid:

≤19

Resistant value of Penicillin G:

≤28

Resistant value of Streptomycin:

≤13

Resistant value of Novobiocin:

≤14

Resistant value of Oxacillin:

≤17

Resistant value of Cephalotin:

≤14

Resistant value of Aztreonam:

≤15

Resistant value of Nalidixic Acid:

≤13

Resistant value of Vancomycin:

≤14

Resistant value of Ciprofloxacin:

≤13

Resistant value of Clindamycin:

≤13

Resistant value of Cefoxitin:

≤13

Resistant value of Bacitracin:

≤8

Intermediate value of Erythromycin:

14-16

Intermediate value of Amoxicillin/Clavulanic Acid:

No value

Intermediate value of Penicillin G:

No value

Intermediate value of Streptomycin:

14-17

Intermediate value of Novobiocin:

15-16

Intermediate value of Oxacillin:

No value

Intermediate value of Cephalotin:

15-17

Intermediate value of Aztreonam:

16-21

Intermediate value of Nalidixic Acid:

14-18

Intermediate value of Vancomycin:

No value

Intermediate value of Ciprofloxacin:

14-17

Intermediate value of Clindamycin:

14-17

Intermediate value of Cefoxitin:

14-19

Intermediate value of Bacitracin:

9-12

Susceptible value of Erythromycin:

≥17

Susceptible value of Amoxicillin/Clavulanic Acid:

≥20

Susceptible value of Penicillin G:

≥29

Susceptible value of Streptomycin:

≥18

Susceptible value of Novobiocin:

≥17

Susceptible value of Oxacillin:

≥18

Susceptible value of Cephalotin:

≥18

Susceptible value of Aztreonam:

≥22

Susceptible value of Nalidixic Acid:

≥19

Susceptible value of Vancomycin:

≥15

Susceptible value of Ciprofloxacin:

≥18

Susceptible value of Clindamycin:

≥18

Susceptible value of Cefoxitin:

≥19

Susceptible value of Bacitracin:

≥13

(Laboratory) Antimicrobial Susceptibility Testing Flashcards

(115 cards)