LEC2 - Specimen Collection

Question 1

in specimen collection, we must Document the following

Answer 1

✓ Patient Identifiers
✓ Sample Identifiers
✓ Location and type
✓ Date and Time of Collection
✓ Relevant clinical information including recent and current antimicrobial the

Question 2

At least how many ml of body fluids

Answer 2

2 mL

Question 3

Transport and process within ____

Answer 3

2 hours

Question 4

Store at how many Celsius if there is a delay in processing

Answer 4

4°C

Question 5

we must store the sample in 4*C Except ____, store at 35
37°C

Answer 5

dermatologic specimens,
Blood,
Bone Marrow
, CSF and sterile tissues
yeasts

Question 6

• Pus and discharge samples are collected using

Answer 6

cotton wool swab

Question 7

only an experienced ___ collects eye

Answer 7

ophthalmologist

Question 8

Intraocular fluid (___ and ____)

Answer 8

vitreous and aqueous

Question 9

if the sample is insufficient - priority to ___

Answer 9

culture

Question 10

corneal scrapings or vitreous humor must be placed directly unto the slide and inoculated onto non inhibitory media in either what shaped pattern

Answer 10

x or c shaped pattern

Question 11

Ear Swab and Scraping, who collects the sample

Answer 11

physician

Question 12

Ear Swab and Scraping

Skin scrapings from the ____ preferred

Answer 12

external auditory canal

Question 13

Deeper ear infections - ___ or experienced physician
should use a speculum to draw specimen.

Answer 13

ENT surgeon

Question 14

collect exudate, pus or debris from the
lesion or inflamed area within the oral cavity

Answer 14

sterile oral swab

Question 15

in oral swab,

___ aids visualization

Answer 15

tongue depressor/spatula

Question 16

Low and High Vaginal Swabs

• ___ be cleared prior to sample collection

Answer 16

mucous discharge

Question 17

Low and High Vaginal Swabs

low vaginal swab (LVS) - who collects the sample

Answer 17

gynecologist or by the patient
herself

Question 18

Low and High Vaginal Swabs

who collects high vaginal swab (HVS)

Answer 18

gynecologist - speculum
and swab stick

Question 19

Adults - two samples (_____ ml each) - aerobic and anaerobic blood culture bottle

Answer 19

10 ml

Question 20

• Children - a single sample (___) - pediatric blood culture bottle

Answer 20

4-10 ml

Question 21

Lumbar Puncture

• a single sample (____ ml CSF) - trained physician

Answer 21

1-10

Question 22

Urine

____ ml midstream urine in a sterile container - first morning urination.

Answer 22

10-20 ml

Question 23

in a catheterized urine sample, what clamp is used

Answer 23

clamp hub of foley’s catheter distally

Question 24

in a catheterized urine sample, we clean the hub using

Answer 24

pyodine and 70% alcohol

Question 25

24-hour urine collection and Foley’s catheter tip samples are ___

Answer 25

not acceptable

Question 26

Surgical collection and punch biopsies are for

Answer 26

skin lesions

Question 27

In biopsy grains - collect aseptically for ___ and ____

Answer 27

smear and culture

Question 28

____ be added to the tissue - prevent drying

Answer 28

Sterile normal saline

Question 29

Exudates, Pus and Drainage are collected by whom

Answer 29

* physician or staff nurse - open wound, ulcer or abscess

Question 30

Exudates, Pus and Drainage send several swab samples for preparation of __

Answer 30

fungal smear and inoculation of culture plates.

Question 31

in an open wound we * Do not swab __areas

Answer 31

dry crusted

Question 32

Ulcer * Prior to specimen collection remove all debris - wash with __

Answer 32

normal saline

Question 33

Ulcer biopsy specimen - we collect in what area

Answer 33

edge of the wound or aspirate with a needle

Question 34

Abscess * Clean skin using _____ -

Answer 34

70% alcohol

Question 35

abscess palpate point of ___

Answer 35

highest fluctuation

Question 36

abscess, we aspirate fluid from the ___

Answer 36

most fluctuant point

Question 37

Abscess obtain sample from ___ of lesion and abscess wall

Answer 37

base

Question 38

Sputum * first morning expectorated sample (optimal) with the following instructions:

Answer 38

* Clean mouth with several rinses of sterile saline or water. * Cough out 2-5 ml of sputum (not saliva) in a wide-mouthed sterile container

Question 39

how many ml of sputum should the patient cough out

Answer 39

2-5 ml

Question 40

how many ml of respiratory specimen should be inoculate to a medium

Answer 40

0.5 mL

Question 41

if the respiratory specimen will be delayed, what will be the temperature requirement

Answer 41

4*C - process must be completed within 2 hrs, if not, store it in 4*C

Question 42

sputum dry cough - perform ____

Answer 42

sputum induction with hypertonic saline nebulization

Question 43

Acceptability of sputum with a Gram-stained smear (pus cells _____, epithelial cells _____)

Answer 43

>25/LPF; <10/HPF

Question 44

Tracheal Aspira collected through an ___

Answer 44

endotracheal tub

Question 45

Tracheal Aspirate subject to the same limitations as ___

Answer 45

sputum specimens

Question 46

lower respiratory tract sample without the need for bronchoscopy.

Answer 46

Non-Directed Bronchoalveolar Lavage

Question 47

suction catheter down the endotracheal tube until resistance is met.

Answer 47

Non-Directed Bronchoalveolar Lavage

Question 48

Inject an aliquot of sterile saline - aspirate at least 1 ml of secretions.

Answer 48

Non-Directed Bronchoalveolar Lavage

Question 49

Skin Scrapings * Clean with ___alcohol

Answer 49

70%

Question 50

skin scales - collected using

Answer 50

sterile petri dish or similar wide-mouthed container or clean, dry piece of paper folded securely with Scotch tape labelled properly.

Question 51

Skin Scraping not yield sufficient material-

Answer 51

swab or Scotch tape could be pressed on the lesion

Question 52

nails are * Examine for ___

Answer 52

damaged, discolored, brittle or dystrophic area

Question 53

Hair and Scalp * _____ - helpful in selecting specimens

Answer 53

Wood’s light

Question 54

Hair and Scalp how to collect hair

Answer 54

Pluck broken or lusterless hairs from periphery of lesion / Scrape scalp from edge of hair loss area

Question 55

_in Piedra infections -how do we collect the sample

Answer 55

cut infected hair with scissors.

Question 56

CSF collected for culture must be filtered through

Answer 56

0.45 um membrane filter attached to a sterile syringe

Question 57

if the csf specimen will not be processed promptly, it must be stored in

Answer 57

room temp or 30*C incubator

Question 58

laboratories that frequently recover dimorphic fungi from blood are encouraged to use the lysis-centrifugation system, ___

Answer 58

the 'isolator'

Question 59

the isolator has been proven optimal for the recovery of

Answer 59

histoplasma capsulatum and other filamentous fungi

Question 60

this system will lyse the rbc and wbc which may contain the microorganism, and centrifugation concentrates the organisms before culturing

Answer 60

the isolator - lysis centrifugation

Question 61

the optimal temp for fungal blood cultures is

Answer 61

30*C for 21 days

Question 62

only what part of the skin lesion must be sampled, and for what reason

Answer 62

only the edge of the skin lesion, the centers often contain nonviable organisms

Question 63

____ is the satisfactory media for the recovery of dermatophytes

Answer 63

mycosel agar which contains chlorampenicol and cycloheximide

Question 64

if the infection with ___ is suspected, olive oil saturated paper disk should be placed on the first quadrant of the agar plate

Answer 64

malassezia furfur

Question 65

cultures for hair, skin, and nail scrapings are incubated at a minimum of how many days and what temp before to be reported as negative

Answer 65

30*C for 21 days

Question 66

vaginal samples must be transported to the lab within ho wmany hrs

Answer 66

24 hrs and kept in moist sterile tubes

Question 67

all tissues should be processed before culturing by ___ and not grounding

Answer 67

mincing

Question 68

bone marrow may be collected in what syringe

Answer 68

heparinized