lecture 1 Flashcards
monoclonal antibodies are used in
pharmaceutical research and therapeutic agents
what is the abbreviation for monoclonal antibodies
mAbs
what does monoclonal mean
produced from a single B-lymphocyte clone and bind the same epitope
what was fist mAb
OKT3
what do OKT3s do
they deplete T cells and serve as an immunosuppressor
immunoglobulin IgG contain what chains
a heavy and light chain
each chain has what domains
light- 1 variable and 1 constant
heavy- 1 variable and 3 constant
where are the disulfide bridges
between the heavy and light chain
where is the antigen binding site
at the end with the variable domains
what does pepsin do to the IgG
it hydrolyzes the peptide back bone and cleaves the IgG into 2
pepsin cleaves the IgG at the hinge peptide into what two molecules
the Fab group and the Fc group
what is the Fab group
2 identical chains, the top half with the variable and first constant domain
what is the Fc group
the bottom half, and it bind the Fc receptor on immune cells
where can the saccharide component be found
in the constant domains on the heavy chains
why are antigens flexable
the loops between the Ig domains
