Lecture 11

Question 1

HSCS give rise to

Answer 1

common lymphoid progenitors

Question 2

CLPs give rise to

Answer 2

Nk, B and T

Question 3

Pro B cells turn into

Answer 3

follicular B cells, marginal zone B cells, B1cells

Question 4

Pro T cells turn inot

Answer 4

alpha beta T cells

lambda delta T cells

Question 5

Ig heavy chain locus opens up

Answer 5

developing B cells

Question 6

alpha beta T cells

Answer 6

TCR beta gene lucs open

Question 7

Who commit to T cell transcrition

Answer 7

Notch 1 and GATA 3

Question 8

Notch activated by being

Answer 8

cleaved

Question 9

enduce B cell development

Answer 9

EBF, E2A, Pax-5

• Rag1 and 2 BCR rearrangment
• surrogate light chins
• the Igalpha dn beta BCR

Question 10

Cytokines stimulate

Answer 10

proliferation

Question 11

produced by stromal cells in thymus drives proliferation of T cell

Answer 11

IL7

Question 12

mutation of gamma chain causes IL2,7,15 to block

Answer 12

cause T cell and NK resulting in immunodeficiency

Question 13

required for NK development

Answer 13

IL15

Question 14

DNA methylation

Answer 14

silences genes

Question 15

miRNA

Answer 15

silence genes

Question 16

modifictioan of histone tails

Answer 16

activate or inactivate

Question 17

can enhance or suppress gene expression

Answer 17

remodeling complex

Question 18

CD4 vs 8

Answer 18

CD4 is suppressed by chromatin modification for CD8 to display

Question 19

miRNA modulate?

Answer 19

T cell during development

Question 20

a key enzyme in miRna

Answer 20

dicer

Question 21

What causes lause of reg T cells?

Answer 21

deletion of dicer

Question 22

loss of Dicer in B cell lineage

Answer 22

block at pro B cell pre B cell transition

Question 23

-miR17-92 plays a key role in?

Answer 23

apoptosis of pre B cells inhibiting Bim

Question 24

miR150 targets ?

Answer 24

a notch receptor that palys improtnat roles in proliferation differentiation and survival of T cells

Question 25

- restriction to VlCl or VhCh | - governs expression of TCR

Answer 25

allelic exclusion

Question 26

3 mechanism for rearrangment

Answer 26

- somatic recomitnation - mRNA splicing - junctional diveristy

Question 27

expression of BCR and TCR initiated by?

Answer 27

somatic recombination

Question 28

epitope specific diversity caused by?

Answer 28

DNA chromosomal rearrangment

Question 29

activating gene 1 and 2 encode enzymes performing recombination of BCR and TCR during VDJ recombintioatn

Answer 29

RAG 1 and 2

Question 30

RAG1 and RAG

Answer 30

restricted to B and T lymphocytes during developmental stages

Question 31

H chains have how many segments/

Answer 31

4 V, D, J, C

Question 32

BCR diversity first

Answer 32

D and J chosen, DNA btw them deleted

Question 33

Second in BCR

Answer 33

J chosen, VDJ btw deleted. C

Question 34

Vh produced?

Answer 34

first... once it is good, goes onto produce Vl

Question 35

second test

Answer 35

rearrangement for Vl

Question 36

VDJ recombination success brings?

Answer 36

promoter sequence closer.... transcrtipion occurs

Question 37

JC lie downstream of the rearrandged VDJ exon sepreated by intron

Answer 37

form RNA transcript

Question 38

splicing out of exons allows

Answer 38

mRNA that can be translated on ribosome to produce antigen receptor

Question 39

kL chain is made up of

Answer 39

35X B 5X J 1X C

Question 40

lambda L

Answer 40

30X B 1XJ 4XC

Question 41

generated at the pts btw the joining gene

Answer 41

junctional diversity

Question 42

Juctional diversity results from?

Answer 42

loss of nucleotides thr action of exonucleases and from the addition of N and P nucleotides

Question 43

P

Answer 43

hairpin loops, can be incorporated in or removed

Question 44

What cleaved DNA hairpin and adds P nucleotides?

Answer 44

RAG

Question 45

addition of N nucleotides aided by?

Answer 45

TdT

Question 46

first check point

Answer 46

after first polypeptide chains of the two chaing Ag receptor is completed

Question 47

checkpoint 2

Answer 47

second polypeptide chain of Ag is completed

Question 48

checkpoints

Answer 48

ensure only those who have successful rearrangement, and eliminate potentially harmful self reactive lyphocytes

Question 49

Pre B and T

Answer 49

Pre B= u | Pre T= beta

Question 50

exibit what to continue going on/>

Answer 50

survival signals, or undergo apoptosis

Question 51

If second poly peptide is bad. what happens to T? B

Answer 51

T=apoptosis | B=rearrangment

Question 52

clonal deletion

Answer 52

getting rid of self recognizing, important mechanism for central tolderance