Lecture 5: CAMs

Question 1

Calcium independent CAMs

Answer 1

Integrins

Immunoglobulin superfamily molecules

Question 2

What is fibronectin

Answer 2

Protein made up of two polypeptide chains cross-linked by disulfide bonds

Question 3

Gap junction function

Answer 3

Composed of molecular pores that allow cells to rapidly exchange ions and small molecules

Question 4

Classes of cadherins and their locations

Answer 4

E-Cadherins–Epithelial tissues
N-Cadherins–Nerve cells
P-Cadherins–Pacenta

Question 5

Laminin has binding sites for

Answer 5

Integrins
Type IV collagen
Entactin
Proteoglycans

Question 6

Classes of Selectins and their functions

Answer 6

P-Selectins–Associated with platelets
E-Selectins–Associated with activated endothelial cells
L-Selectins–Associated with leukocytes

Question 7

Function of integrins in hemidesmosomes

Answer 7

Link the basil domain of the cell to the extracellular matrix via the intercellular tonofilamints (intermediate filaments such as keratin)

Question 8

Components of basil lamina

Answer 8

Laminin
Fibronectin
Type IV collagen
Entactin
Proteoglycans

Question 9

Selectin function

Answer 9

Selectins are involved in the movement of leukocytes from blood to tissues (extravasation)

Question 10

Members of immunoglobulin superfamily and function

Answer 10

N-CAM- Neural adhesion molecues
I-CAM1- Facilitates transendothelial migration of leukocytes
I-CAM2

Question 11

Nonclassical cadherins

Answer 11

Include desmocollins and desmogleins

Found in desmosomes (macula adherens)

Question 12

E-Cadherins

Answer 12

Most common type

Loss of E-Cadherin is associated with invasive behavior of tumor cells

Question 13

Basement membrane components

Answer 13

Basil lamina

Reticular lamina

Question 14

Hemidesmosomes structure/function

Answer 14

Link basal domain to basal lamina via intermediate filaments which associate with intracellular plaques which associate with integrin and anchoring filaments (laminin 5)

Question 15

What are integrins

Answer 15

Glycoproteins that are mainly involved in cell-extracellular matrix interactions

Question 16

Gap junctions are composed of ____ , which form what

Answer 16

Connexons, form hydrophillic intercellular channel between adjacent cells

Question 17

Integrins bind what together

Answer 17

Extracellular matrix molecules and the cytoskeleton

Question 18

Three chains of laminin

Answer 18

Alpha
Beta
Delta

Question 19

Zonula-type of junction, function, filament association

Answer 19

Adheren or occluden
Travel all the way around the circumference of the cell and link all neighboring cells to the central cell (belt-like)
Associated with intracellular actin

Question 20

What are Catenins

Answer 20

Small proteins that link the cytoplasmic end of a cadherin with the cytoplasmic actin

Question 21

Focal adhesions

Answer 21

Spots that anchor the cell to the extracellular matrix via integrins

Question 22

What is the CRD

Answer 22

Carbohydrate recognition domain-binding site for carbohydrates on selectins
Calcium is required for binding

Question 23

Basil lamina produced by

Answer 23

Epithelium

Question 24

Zonula occluden structure/function

Answer 24

Belt-like structure preventing paracellular transport
Associated transmembrane proteins include occludens and claudins. They attach intracellularly to zonula occluden proteins ZO-2 & ZO-3, which attach to actin

Question 25

Reticular lamina produced by

Answer 25

Connective tissue

Question 26

Why are selectins classified as lectins

Answer 26

Because they bind to carbohydrates

Question 27

Adheren function and location

Answer 27

Anchor cells together and reinforce the physical integrity of tissues and cells that make up tissues Found on basolateral domain of epithelial cells

Question 28

Calcium dependent CAMs

Answer 28

Cadherins | Selectins

Question 29

Connexons are composed of

Answer 29

6 Connexins

Question 30

Cadherin proteins are anchored by

Answer 30

Intracellular plaques

Question 31

Adherens characterized by

Answer 31

Intercellular spaces which are filled with cadherin proteins desmogleins and desmocollins

Question 32

Macula- type of junction, function, filament association

Answer 32

Adheren (often referred to as desmosomes or hemides) A "spot" or snap-like junction Associated with intracellular intermediate filaments (tonofilaments)

Question 33

Pemphigus foliaceus

Answer 33

Autoantibody-mediated blistering disease in which antibodies against desmoglein-1 cause a loss of adhesion of keratinocytes in the superficial layers of epidermis

Question 34

Extracellular domain of integrins bind to what

Answer 34

Extracellular matrix molecules such as fibronectin and laminin

Question 35

Categories of junctional complexes

Answer 35

Adherens Occludens Gap junctions

Question 36

Fibronectin has binding sites for

Answer 36

Integrins Collagen Heparin (proteoglycan) Fibrin

Question 37

Major component of basil lamina

Answer 37

Laminin

Question 38

Forms of fibronectin

Answer 38

Cellular (produced by fibroblasts) | Plasma

Question 39

Occluden function

Answer 39

Establish impermeable barrier between adjacent cells Maintains concentration differences between environments on either side of epithelial sheets