maldi

Question 1

maldi method to ID bacterial via

Answer 1

ribosomal protein patterns

Question 2

maldi mass spec technique works by separating ions by

Answer 2

mass to charge ratio

Question 3

ion source

Answer 3

maldi

Question 4

mass analyzer

Answer 4

TOF- time of flight

Question 5

Maldi Principle: ionization process that involves a laser striking a matrix of small molecules to make those molecules into a gas phase

Answer 5

without fragmenting or decomposing them

Question 6

solid surface is called

Answer 6

target

Question 7

What is added to the target?

Answer 7

• Alpha cyano-4 hydroxycinnamic acid
• This extracts proteins from organism while on the target

Question 8

2 times of flight modes

Answer 8

linear and reflector

Question 9

linear mode- molecules fly

Answer 9

flight time determines the molecular mass

Question 10

maldi can’t differentiate what organisms

Answer 10

E.coli from shigella

S. Pneumo and S. mitis

Question 11

product of PCR called

Answer 11

amplicon

Question 12

biggest problem with PCR

Answer 12

contamination with nucleic acids

Question 13

3 steps of PCR

Answer 13

denaturation, annealing and extension

Question 14

how many primers needed for PCR

Answer 14

2- need same annealing temps

Question 15

Denaturation takes place at

Answer 15

95 degrees C

Question 16

Annealing takes place as primers attach at

Answer 16

55 degrees C

Question 17

Elongation step where you have thermostable DNA polymerase (Taq polymerase) takes place at

Answer 17

72 degrees C

Question 18

multiplex PCR

Answer 18

• Allows multiple primers to be used to target different components in a single reaction mixture

Question 19

Peptide Nucleic Acid Fluorescence In Situ Hybridization

Answer 19

• Detect presence of bacteria/yeast straight from blood culture bottle
• Use fluoresce to detect combining of probe and target DNA/RNA