Mammalian genome modification techniques Flashcards
(9 cards)
1
Q
Where are ES cells derived from?
A
Inner cell mass of blastocysts
2
Q
Result of ES cells being injected into a blastocyst?
A
they can integrate and contribute to development
3
Q
What do ES cells support high rates of?
A
homologous recombination
4
Q
First reporting of ES cells?
A
1981 (mice), 1998 (humans)
5
Q
What is the goal of ES cell gene targeting methodology?
A
to harness homology-dependent recombination to
make targeted, prescriptive (non-random) genome changes
6
Q
Possible improvement to be made to transgenesis?
A
Want to make it easier to direct where in the genome the transgene will go
7
Q
What is TV?
A
targeting vector DNA construct
8
Q
How are TV integration events selected for?
A
positive selection (eg Neomycin resistance)
9
Q
Additional elements of a targeting vector?
A
