Stem cells III Flashcards
(21 cards)
What are some pluripotency markers?
Oct4, Nanog, SSEA1, Lin28
How is pluripotency looked for?
Immunostaining for pluripotency markers
Where are Oct4 and Nanog localised to?
The nucleus
Where are SSEA1 and Lin28 localised to?
Cell surface
What are Oct4 and Nanog?
TFs
What is needed for ES cells to contribute to whole organism development?
Host blastocyst
How can you test the pluripotency of ES cells?
Take ES cells from agouti/brown mice
Inject them into the blastocyst of albino mice
Look at coat colour–> should be brown and albino
What is formed as a result of injecting ES cells from a brown mouse into an albino blastocyst?
Chimeric mice (2 coat colours)
Length of ES cell cycle?
Short w/ minimal growth phases
Difference in division speed between adult and ES cells?
ES cells divide v rapidly
What do embryoid bodies resemble?
The early embryo
What can be done with embryoids?
Directed to differentiate along diff lineages
What type of cell line do HepG2 cells follow?
Liver cells
What line does OP9 cells belong to?
Haematopoietic cells
Examples of chemicals that can induce specific lineages of ES cells?
Retinoic acid, dimethylsulfoxide,
Example of a pathway that can be inhibited to produce lineage specific differentiation?
SHH
General groups of things that can direct ES development?
Growth factors, chemicals, altering pathways, specific media
What is nestin a marker for?
Neural precursors and pancreatic progenitor cells
How are nestin-positive cells made from ES cells?
ES cells into a media containing insulin, transferrin, fibronectin
What media is added to a nestin +ve cell to make it neuronal?
Neuro2 medium
Basic FGF, lamnin
then remove basic FGF
