MHC

Question 1

Role of MHC / HLA molecules:
- main role

Answer 1

is during Antigen Presentation to T cells

Question 2

• play a pivotal role in development of both humoral and cellular immunity

Answer 2

MHC/ HLA

Question 3

MHC/ HLA

• during transplants:
  used to determine whether a transplanted or to be transplanted tissue is:

Answer 3

• histocompatible and thus accepted
• recognized as foreign and rejected

Question 4

MHC/ HLA

• may be involved during:

Answer 4

• Graft Rejections
• Autoimmune Diseases
• Paternity Tests
• Transfusion Reactions

Question 5

Why are MHC/ HLA Gene System
- Polymorphic:

Answer 5

there are so many possible alleles at each location to increase chances of interacting with diverse self and non-self antigens/immunogens

Question 6

MHC/ HLA

Coded in the…
Except…

Answer 6

short arm of chromosome 6 band 21 (6p21)

Beta-2 in chromosome 15

Question 7

MHC/ HLA gene system

Divided into 3 categories (classes)

Answer 7

• Class I
• Class Il
• Class III

Question 8

MHC

Class I

Answer 8

ABC

Question 9

MHC CLASS II

Answer 9

D:PQR

Question 10

MHC CLASS III

Answer 10

Complement proteins
Cytokines
Tumor necrosis factor

Question 11

Expressed on cell surface

Answer 11

MHC CLASS I and CLASS II

Question 12

MHC Class I

Answer 12

(HLA-A, HLA-B, HLA-C)

Question 13

MHC Class I

Answer 13

(HLA-A, HLA-B, HLA-C)

Question 14

MHC Class | (HLA-A, HLA-B, HLA-C)

• expressed by…
• highly found on…
• Low or undetectable on… (4)

Answer 14

ALL nucleated cells

Lymphocytes & Myeloid Cells

hepatocytes, muscle cells, sperm cells, neural cells

Question 15

What MHC/ HLA
IS expressed the lowest

Answer 15

HLA-C

Question 16

What MHC/ HLA

(______ and ______ are more important!)

Answer 16

HLA-A and HLA-B

Question 17

Structure of MHC Class I

Answer 17

glycoprotein dimer:

2 non-covalently bonded polypeptide chains: - Alpha - Beta

Question 18

Structure of MHC class I

ALPHA chains
- MW: ____
- folded into 3 domains:

-_______ anchored to the cell membrane

Answer 18

44,000

• Alpha 1
• Alpha 2
• Alpha 3

polypeptide chain

Question 19

Structure of MHC Class I
BETA chain
- MW:_____
- coded on______
- Beta…
- stabilize folded____
- not polymorphic: why?..

Answer 19

12,000

chromosome15

B2 microglobulin

alpha chain

every class I molecule contains the same B2 microglobulin

Question 20

MHC Class III

HLA proteins

Answer 20

BF, C2, C4, TNF

Question 21

MHC CLASS I

MHC CLASS II

Chain Structure

Answer 21

3 alpha, 1 beta2-microglobulin

2 alpha and 2 beta

Question 22

MHC CLASS I
MHC CLASS II
MHC CLASS III

Found on

Answer 22

All nucleated cells

APCS

Blood and other secretions

Question 23

MHC CLASS I
MHC CLASS II

Example of infectious agent

Answer 23

Viruses, Tumor, Certain Parasitic Antigens, Intracellular bacteria

Bacteria

Question 24

MHC CLASS I
MHC CLASS II
MHC CLASS III

Antigen Presentation

Answer 24

CD8+
CD4+
No role in antigen presentation

Question 25

- molecular basis for T-cell discrimination of self from non-self

Answer 25

Human Leukocyte Antigens

Question 26

HLA - Name was given by… - discovery of antibody response to…

Answer 26

French Scientist (Jean Dausset) circulating WBCs

Question 27

- determine whether transplanted tissue is histocompatible and thus accepted or recognized as foreign and rejected - Antigen presentation

Answer 27

Major Histocompatibility Complex

Question 28

- in general: Gene system which plays a key immunologic role.

Answer 28

MHC

Question 29

Structure of MHC Class I ALPHA 1 and 2 - serves as… - able to hold…

Answer 29

antigen binding site 8 - 11 amino acids

Question 30

Structure of MHC Class I ALPHA 3 - detected by…

Answer 30

CD8 on Cytotoxic cells

Question 31

MHC CLASS I NONCLASSICAL GENE

Answer 31

EFG

Question 32

Nonclassical antigens Expression

Answer 32

E - cell surface F - cell surface G - nit expressed on cell surface

Question 33

Nonclassical antigens Function

Answer 33

E - expressed on fetal trophoblast cells during first trimester of pregnancy protection of placental tissue from NK cells F - Unknown G - expressed on fetal trophoblast cells during first trimester of pregnancy protection of placental tissue from NK cells

Question 34

MHC Class II (HLA-DP, HLA-DQ, HLA-DR) •expressed by____ •_______: highest expression

Answer 34

APCs Dendritic cells

Question 35

MHC Class II (HLA-DP, HLA-DQ, HLA-DR) : lowest :is expressed at the highest level

Answer 35

HLA-DP HLA-DR

Question 36

MHC Class II (HLA-DP, HLA-DQ, HLA-DR) is expressed at the highest level accounts for about ½ of Class Il molecules of a particular cell

Answer 36

HLA DR

Question 37

MHC Class II (HLA-DP, HLA-DQ, HLA-DR) : the most highly polymorphic

Answer 37

HLA-DR Beta

Question 38

Structure of MHC Class II - Consist of… - ALPHA - BETA - _______anchored in the cell membrane

Answer 38

two noncovalently bonded polypeptide chains, both of which is encoded at the chromosome 6 Both chains are

Question 39

Structure of MHC Class II ALPHA chains - MW: - folded into 2 domains: - both:

Answer 39

34,000 - ALPHA 1 - ALPHA 2 serves to as recognition sites by T helper cells via the CD4 surface marker

Question 40

Structure of MHC Class II ALPHA 1 and BETA 1 domains - can hold up to…

Answer 40

- antigen-peptide-binding site 13 - 18 aa

Question 41

MHC Class II: Nonclassical Function DM DN DO

Answer 41

Loads peptides onto class Il Unknown Modulates antigen binding