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Question 1

Parts of Microscope and Function
Magnifying Parts

Answer 1

Eyepiece (ocular lens)

Objective lenses

Question 2

The part that is looked through
at the top of the compound microscope. Eyepieces typically have a
magnification between 5x & 30x.

Answer 2

Eyepiece (ocular lens)

Question 3

provides the most powerful magnification,
with a whopping magnification total of 1000x when combined with a 10x
eyepiece. (special low auto-fluorescence oil like cedar wood oil)

Answer 3

oil immersion

Question 4

Mechanical Parts

Answer 4

Arm

Nosepiece

Base:

Coarse and fine adjustment controls

Stage height adjustment

Specimen or slide

Stage or Platform

Stage clips or mechanical stage

Question 5

Supports the microscope head and attaches it
to the base.

Answer 5

Arm:

Question 6

Holds the objective lenses & attaches
them to the microscope head. This part rotates to
change which objective lens is active.

Answer 6

Nosepiece

Question 7

Bottom base of the microscope that houses
the illumination & supports the compound
microscope.

Answer 7

Base:

Question 8

Adjusts the
focus of the microscope. These knobs increase or
decrease the level of detail seen when looking at the
slide or specimen through the eyepiece of the
compound microscope.

Answer 8

Coarse and fine adjustment controls

Question 9

Adjusts the position of
the mechanical stage vertically & horizontally. It is
important to adjust these knobs so that the
objective lens is never coming into contact with
the slide or specimen on the stage.

Answer 9

stage height adjustment.

Question 10

hold the specimen in place
along with slide covers for viewing.

Answer 10

Specimen or slide

Question 11

which the specimen or slide
are placed. The height of the mechanical stage is
adjustable on most compound microscopes.

Answer 11

Stage or Platform

Question 12

hold the slide in
place on the mechanical stage.

Answer 12

Stage clips or mechanical stage

Question 13

lluminating Parts

Answer 13

Aperture - Disc or Iris Diaphragm

Abbe Condenser

Mirror

Illumination

Bottom Lens or Field Diaphragm

Parfocal

Parfocalling

Field of view microscope-

Working distance

Question 14

Circular opening in the stage
where the illumination from the base of the compound
microscope reaches the platform of the stage.

Answer 14

Aperture - Disc or Iris Diaphragm

Question 15

This lens condenses the light from the base
illumination and focuses it onto the stage. This piece of the
compound microscope sits below the stage & typically acts as a
structural support that connects the stage to arm or frame of the
microscope.

Answer 15

Abbe Condenser:

Question 16

Reflects light into the base of the microscope. Earlier
microscopes used mirrors that reflected light into the base of the
microscope instead of halogen bulbs as their source of
illuminationV

Answer 16

Mirror

Question 17

Light used to illuminate the slide or specimen from
the base of the microscope. Low voltage halogen bulbs are the
most commonly used source of illumination for compound
microscopes.

Answer 17

Illumination

Question 18

Knob used to adjust the amount
of light that reaches the specimen or slide from the base
illumination.

Answer 18

Bottom Lens or Field Diaphragm

Question 19

refers to objectives that can be changed
with minimal or no refocusing.

Answer 19

Parfocal

Question 20

The area you see under
the microscope for a particular magnification.

Answer 20

Field of view microscope-

Question 21

-distance between the front
edge of the objective lens and the specimen
surface (with the surface of the cover glass in
case of the cover glass objective lens) when the
specimen is focused.

Answer 21

Working distance

Question 22

Bacillus subtilis

Answer 22

rod-shaped and Gram-positive

Question 23

Spirillum sp

Answer 23

Spiral shaped, gram-
negative

Question 23

PROTOZOANS

Answer 23

Amoeba proteus

Paramecium

Question 24

Streptococcus pyogenes

Answer 24

short chains, diplococci and single cocci
Gram-positive bacteria

Question 25

staphylococcus-aureus

Answer 25

Gram-postive, cocci,
grape-like

Question 26

ALGAE

Answer 26

Spirogyra

Volvox

Question 27

is movement caused by the
molecules in a liquid striking/hitting against
the particles of an object (eg. Microorganisms)
leading to shaking and vibration

Answer 27

Brownian motility

Question 28

is the deliberate/ self-propelled
movement of the organism using its own
features such as flagella,pili/fimbriae.

Answer 28

True motility

Question 29

Gram Positive Bacteria:

Answer 29

Actinomyces, Bacillus, Clostridium,
Corynebacterium, Enterococcus, Gardnerella, Lactobacillus,
Listeria, Mycoplasma, Nocardia, Staphylococcus, Streptococcus,
Streptomyces

Question 30

Gram Negative Bacteria:

Answer 30

Escherichia
coli (E.coli),

Salmonella

Shigella

Enterobacteriaceae

Pseudomonas

Moraxella

Helicobacter

Stenotrophomonas

Bdellovibrio,

acetic acid bacteria,

Legionella etc