Flashcards in Microbiology Deck (260)
What samples can be used for diagnosis?
green viral swab, black charcoal swab, CSF, stool
What tests count WCC quickly?
What blood tests can be done?
FBC, blood film and yellow top for EBV serology
How long does it take for blood results to be available?
film - hours
pcr - next day
acute EBV serology - 1-2 days
Management of glandular fever?
supportive therapy, avoid contact sport for 6 weeks to avoid splenic rupture
What 3 markers can HIV tests detect in the blood?
antibody, antigen p24, HIV RNA
What happens if HIV test is negative?
may not be in window period, so screen again after window period
What happens in HIV test is positive?
confirm test inn the lab with a different assay
Treatment of CMV?
Causes of UTI?
sexual intercourse, catheterisation, enlarged prostate, renal tract tumours, renal stones
What investigations are need in UTI?
mid stream urine, direct microscopy for pyuria, culture (which can show significant bacteriuria, doubtful significance or a contaminant), sensitivity testing (to find appropriate antibiotics)
What is a skin organisms that can colonise the perineium and seen in UTI?
staphylococcus saprophyticus (gram positive cocci)
What do leucocytes and nitrates in urine sample suggest?
How can gram negative bacilli appear on a CLED agar?
yellow (lactose fermenting)
white (non lactose fermenting)
What antibitoics is E.coli pyelonephritis resistant and sensitive to?
r = amoxicillin
s = co amoxiclav
Why can't catheter bag urine be used for urinalysis results?
because all catheters have bacteria and white cells in, even without an infection
Benefits of CLED agar?
promotes UTI pathogens
distinguished lactose and non lactose fermenting
prevents proteus swarming
When should you treat asymptomatic bactriuria?
in pregnant women
What is erysipela?
intradermal skin infection
What causes erysipela?
group A beta haemolytic streptococcus and staphylococcus aureus
What is cellulitis?
a subcutaneous skin infection
What causes cellulitis?
group A beta haemolytic streptococcus (occasionally B, C, G) and staphylococcus aureus
In who are atypical organisms causing skin infections common?
IV drug users and immunocompromised
What organisms commonly cause skin soft tissue abscess?
What organisms commonly causes lung abscesses?
staphylococcus aureus, kelbiella pneumoniae, anaerobic organisms, mycobacterium tuberculosis
What organisms commonly cause liver abscesses?
gram negative, strep.milleri, anaerobes, entamoeba histolytica in tropic
What organisms commonly cause kidney abscesses?
e.coli and klebsiella
What organisms commonly cause bowel abscesses?
gram negative, anaerobes, strep.milleri
How does impetigo present?
itchy, growing in size spot on the face, it weeps and crusts