microscopic exam(aubf) Flashcards
(41 cards)
Used for routine urinalysis
BRIGHT-FIELD
Enhances visualization ofelements with low refractive indices
PHASE-CONTRAST
Aids in identification of cholesterolin oval fat bodies, fatty casts, crystals and lipids
POLARIZING
Aids in identification of Treponemapallidum
DARK-FIELD
Allows visualization ofnaturally fluorescent microorganism or thosestained by fluorescent dye
FLUORESCENCE
Produces 3Dmicroscopic image and layer-by-layer imaging ofa specimen
INTERFERENCE-CONTRAST
Crystal violet and Safranin O
STERNHEIMER - MALBIN
- Delineates structure and contrasts colors of thenucleus and cytoplasm
STERNHEIMER - MALBIN
Identifies WBCs, epithelial cells and casts
STERNHEIMER - MALBIN
- Enhances nuclear detail- - Differentiates WBCs and RTE cells
TOLUIDINE BLUE
- Identifies free fat droplets and lipid-containingcells and casts
OIL RED O and SUDAN III
- Triglycerides and neutral fats stain orange – red
OIL RED O and SUDAN III
- Cholesterol does not stain but is capable of polarization
OIL RED O and SUDAN III
Stains structures containing iron
PRUSSIAN BLUE STAIN
Identifies yellow–brown granules of hemosiderinin cells and casts
PRUSSIAN BLUE STAIN
Differentiates gram – positive from gram –negative bacteria
GRAM STAIN
Identifies bacterial casts
GRAM STAIN
Methylene blue and Eosin Y stain eosinophilicgranules
HANSEL STAIN
Identifies urinary eosinophils
HANSEL STAIN
Non-nucleated biconcave disks (7mm)
RED BLOOD CELLS
- Crenated in hypersthenuric urine
RBCs
- GHOST CELLS in hyposthenuria
RBCs
Sources of error
● Yeasts
● Air bubbles
● Oil droplets
● Calcium oxalate crystals
are the predominantly seen WBCs
neutrophils