microscopic exam(aubf) Flashcards

(41 cards)

1
Q

Used for routine urinalysis

A

BRIGHT-FIELD

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2
Q

Enhances visualization ofelements with low refractive indices

A

PHASE-CONTRAST

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3
Q

Aids in identification of cholesterolin oval fat bodies, fatty casts, crystals and lipids

A

POLARIZING

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4
Q

Aids in identification of Treponemapallidum

A

DARK-FIELD

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5
Q

Allows visualization ofnaturally fluorescent microorganism or thosestained by fluorescent dye

A

FLUORESCENCE

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6
Q

Produces 3Dmicroscopic image and layer-by-layer imaging ofa specimen

A

INTERFERENCE-CONTRAST

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7
Q

Crystal violet and Safranin O

A

STERNHEIMER - MALBIN

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8
Q
  • Delineates structure and contrasts colors of thenucleus and cytoplasm
A

STERNHEIMER - MALBIN

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9
Q

Identifies WBCs, epithelial cells and casts

A

STERNHEIMER - MALBIN

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10
Q
  • Enhances nuclear detail- - Differentiates WBCs and RTE cells
A

TOLUIDINE BLUE

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11
Q
  • Identifies free fat droplets and lipid-containingcells and casts
A

OIL RED O and SUDAN III

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12
Q
  • Triglycerides and neutral fats stain orange – red
A

OIL RED O and SUDAN III

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13
Q
  • Cholesterol does not stain but is capable of polarization
A

OIL RED O and SUDAN III

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14
Q

Stains structures containing iron

A

PRUSSIAN BLUE STAIN

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15
Q

Identifies yellow–brown granules of hemosiderinin cells and casts

A

PRUSSIAN BLUE STAIN

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16
Q

Differentiates gram – positive from gram –negative bacteria

A

GRAM STAIN

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17
Q

Identifies bacterial casts

18
Q

Methylene blue and Eosin Y stain eosinophilicgranules

19
Q

Identifies urinary eosinophils

20
Q

Non-nucleated biconcave disks (7mm)

A

RED BLOOD CELLS

21
Q
  • Crenated in hypersthenuric urine
22
Q
  • GHOST CELLS in hyposthenuria
23
Q

Sources of error

A

● Yeasts
● Air bubbles
● Oil droplets
● Calcium oxalate crystals

24
Q

are the predominantly seen WBCs

25
GLITTER CELLS in hypotonic urine exhibits Brownian movement
neutrophils
26
is the increase inurinary WBC and indicates the presence of an infection or inflammation
pyuria
27
Increased in Acute Interstitial Nephritis
WBCs
28
Largest cell in the sediment with abundant,irregular cytoplasm and prominent nuclei
EPITHELIAL CELLS Squamous
29
Originate from the linings of the vagina and female urethra and the lower portion of the male urethra
EPITHELIAL CELLS Squamous
30
CLUE CELLS are epithelialcells studded with bacteria ● Bacterial vaginosis: G.vaginalis
EPITHELIAL CELLS Squamous
31
Spherical, polyhedral orcaudate with distinct, centrallylocated nucleus
EPITHELIAL CELLS Transitional (Urothelial)
32
- Most clinically significant ofthe epithelial cells
EPITHELIAL CELLS Renal Tubular (RTE)
33
Rectangular, polyhedral,cuboidal or columnar with aneccentric nucleus
EPITHELIAL CELLS Renal Tubular (RTE)
34
>_ RTE/HPF indicates necrosis of the renal tubules with thepossibility of affecting overall renalfunction
2
35
- lipid-containing RTE cells
OVAL FAT BODIES
36
cholesterol in oval fat bodies
maltese cross formation
37
nephrotic syndrome in oval fat bodies
lipiduria
38
- RTE cells with nonlipid- containingvacuoles - Acute Tubular Necrosis
BUBBLE CELLS
39
- Increased in number produce (+)nitrite & pH above 8
bacteria
40
Can be indicative of lower or upper UTI
bacteria
41
Present as a result of vaginal, urethral, externalgenitalia, or collection-containercontamination. - Not present in sterile collection
bacteria