remedial monday Flashcards by Collins Colocado

this type of culture medium contains inhibitory agents for isolation of specific microorganism groups

SELECTIVE MEDIUM

How well did you know this?

Not at all

Perfectly

what is the optimal cultivation time for growing microbes

18-24 hours

How well did you know this?

Not at all

Perfectly

optimal temperature for growing bacteria

35-37 C

How well did you know this?

Not at all

Perfectly

this is the cause of the acid fastness of acid fast bacteria

mycolic acid

How well did you know this?

Not at all

Perfectly

this microscope is used to view ultra structures of a specimen

TRANSMISSION ELECTRON MICROSCOPE

How well did you know this?

Not at all

Perfectly

this microscope is used to observe the detailed surface structures of microorganisms

scanning electron microscope

How well did you know this?

Not at all

Perfectly

this technique in AST uses an antibiotic disk on MHA

kirby-bauer technique

How well did you know this?

Not at all

Perfectly

this is a national resource for molecular biology information

NCBI

How well did you know this?

Not at all

Perfectly

this virulence factor of s.aureus activates complement, IL-1, and is chemotactic to PMNs

Peptidoglycan

How well did you know this?

Not at all

Perfectly

this virulence factor of s.aureus has affinity to the Fc receptor of IgG and complement

Protein A

How well did you know this?

Not at all

Perfectly

this cytotoxin in s.aureus is associated with panton valentin leukocidin

cytotoxin gamma

How well did you know this?

Not at all

Perfectly

this toxin is responsible for toxic shock syndrome in s.aureus infection

TSST-1 / ENTEROTOXIN F

How well did you know this?

Not at all

Perfectly

this virulence factor is also known as spreading factor in s.aureus

hyaluronidase

How well did you know this?

Not at all

Perfectly

this virulence factor in s. aureus is lethal to PMNs and responsible for severe cutaneous infections and necrotizing pneumonia

Panton Valentin Leukocidin

How well did you know this?

Not at all

Perfectly

bullous exfoliative dermatitis that can progress to ritter disease

scalded skin syndrome

How well did you know this?

Not at all

Perfectly

these s.aureus enterotoxins cause staphylococcal food poisoning

A, D, B

How well did you know this?

Not at all

Perfectly

THIS BACTERIA IS A COMMON CAUSE OF HAI UTIs and prosthetic valve endocarditis

s. epidermis

How well did you know this?

Not at all

Perfectly

this bacteria is associated with UTIs IN SEXUALLY ACTIVE YOUNG WOMEN

s. saprophyticus

How well did you know this?

Not at all

Perfectly

this is the special plating medium for staphylococcal specimens.

5% CNA

How well did you know this?

Not at all

Perfectly

this bacteria has colonies that are small tommesium and grayish white that stick to the agar

s. epidermis

How well did you know this?

Not at all

Perfectly

this is the selective/inhibitory agent of CNA

naladixic acid

How well did you know this?

Not at all

Perfectly

this is the selective agent in Mannitol salt agar

7.5% sodium chloride

How well did you know this?

Not at all

Perfectly

mannitol fermenters in MSA produce what color

yellow colonies with yellow halo

How well did you know this?

Not at all

Perfectly

positive result of the catalase test

effervescence

How well did you know this?

Not at all

Perfectly

you performed an oxidation-fermentation test on a microorganism. The results show a green closed tube but a yellow open tube. What is the organism?

oxidizer

this test yields a blue to purple color after the oxidase enzyme reacts with the oxidase reagent and cytochrome C to create indophenol

Microdase Test

if the DNase test is positive, what result should you expect?

clearing around the growth

what does the tube coagulase test detect?

free coagulase

this staphylococcus species is resistant to novobiocin

s. saphrophyticus

these are the ribosomal RNA sequences for detecting bacteria

16s and 30s

primary mechanism of resistance to beta lactam antibiotics

production of altered PBP

antibiotic used in MRSA screen agar

oxacillin

mechanism in macrolide resistance in bacteria

efflux pump

what does it mean if an organism is capnophilic

requires increased CO2 for growth

this streptococcus is nto considered normal microbiota and can cause rheumatic or scarlet fever

S. Pyogenes / Group A Strep

O2 labile hemolysin responsible for hemolysis under anaerobic conditions

streptolysin O

toxin responsible for non bullous impetigo

epidermolytic toxin

this virulence factor in group A strep causes the molecule to resist phagocytosis and plays a role in adherence to mucosal cells

M Protein

this impetigo does not form a honey coloured crust with no erythema or edema surrounding it

Bullous Impetigo

this illness is characterized by a rash on the face and upper trunk with a strawberry tongue in active infection

scarlet fever

when infected with s.pneumoniae, what is the hallmark color or the sputum

rust colored

this strep group grows in the presence of bile, hydrolyze esculin, grows in 6.5% NaCl and PYR - positive

enterococci

this streptococcal vaccine is recommended for infants, young children, and all adults younger than 65 years

PCV 13

in this disease, the penis has yellowish purulent discharge and dysuria

Gonorrhea

this occurs when meningitis becomes fulminant and spreads rapidly causing disseminated intravascular coagulation, septic shock or haemorrhage in adrenal glands

Waterhouse-Friderichsen Syndrome

common media for initial and general plating of streptococci

5% SBA

this indicates a positive result for s. agalactiae in CAMP test

arrowhead hemolysis

after performing optochin solubility test on streptococci, the result is resistant. What is the speculated microorganism?

Viridians Strep

When a patient undergoes a serologic test for streptococcus infection, what are you searching for?

Anti-Streptolysin O Titer / Streptolysin O

This is the initial plating media for Neisseria species

5% SBA and CAP

This is diagnostic of gonococcal disease when performing direct gram staining of urethral discharge

appearance of gram negative diplococci inside PMNs

this neisseria species ferments glucose and maltose

N. Meningitis

this indicates the presence of cytochrome oxidase activity in microorganism

oxidase test

give the reagent used in the superoxol test.

30% H2O2

default shape of bacteria

cocci

carried by the TOX gene from a beta phage.

Diphtheria Toxin

this is how diphtheria toxin genes are integrated into the chromosome so the bacteria becomes pathogenic

Lysogenic Conversion

this is the effect C. diphtheriae toxin on cells

inhibits protein synthesis

Pharyngitis is often characterized by

exudative pseudomembrane

in tinsdale medium and CTBA, C. diphtheriae produces these characteristics

Brown to Black Colonies with Gray to Brown Halos

this skin test is used to determine the susceptibility of a person to diphtheria toxin

schick test

this virulence factor in listeria enables cell to cell spreading

ACT A

drug of choice for listeria CNS infection

SXT

this technique has samples inoculted in a nutrient broth incubated for several weeks at 4C

Cold enrichment technique

motility of listeria

tumbing end to end motility

listeria growth in SIM

inverted christmas tree/umbrella growth

this serotype causes most foodborne infections

in PCR detection for Listeria, what is targeted by primers

Genes for virulence factors or RNA sub-unit genes

give the clinically significant bacillus species

anthracis, cereus, mycoides, thurginensis

these are painless, small dark areas that appear in the center of the ring with a depressed black necrotic central area

Malignant pustule / black eschar

this is also known as wool sorter's disease

inhalation anthrax

heat stable proteolysis resistant toxin in B. cereus that causes emetic symptoms

cerulide

toxin that is toxic to insects and nematodes injected into corn

parasporal crystalline inclusions

the size of this gram positive rod is >1 um with no endospore swelling

anthracis cereus mycoides thurginensis

this agar is best and selective for G+ positive bacteria

pea agar

this prevents growth of yeast and filamentous fungi in CNA

amphotericin B

in 5% SBA, small to large colonies with various appearances surrounded with a small zone of beta hemolysis are seen. What is the speculated organism?

Arcanobacterium

this bacteria is often found in clue cells, large squamous epithelia with numerous short rods

G. Vaginalis

what is the hallmark growth of E. rhusiopathae in SIM

Brush growth with blackening in medium

most common drug of choice for G. Vaginalis

Metronidazole

this organism has a greenish metallic sheen in EMB

E. Coli

this microbe is known to be very biochemically inert

shigella

what species are truly pathogenic in family enterobacteriaceae?

salmonella shigella yersinia

this is associated with hemorrhagic diarrhea, colitis, and HUS.

E. coli 0157:H7

Case study: TSIA results were A/A with gas production. IMV:C results are ++-- What is the bacteria causing symptoms in the patient?

E.coli