MLT 259 BB Study Guide

Question 1

List antibodies that may cause HDN

Answer 1

ABO, RH, Kell, Duffy

Question 2

List antibodies that may cause delayed HTR

Answer 2

Kidd

Question 3

If R2R2 patient has a 3+ reaction and is compatible with R1r donor, what antibody is present?

Answer 3

anti-E

Question 4

How are cold agglutinates and rouleaux excluded from testing?

Answer 4

Cold - absorption

Roul - saline replacement

Question 5

List antibodies, detected at different phases

Answer 5

AHG - Kell, Duffy, Kidd, MNS, Lutheran
IS - Lewis, MN, P1
37 - Rh, MN

Question 6

A. interval between donations

Answer 6

a. 8 weeks

Question 7

B. Hgb

Answer 7

b. greater than or equal to 12.5 g/dL

Question 8

C. Hct

Answer 8

c. greater than or equal to 38%

Question 9

D. total volume of blood drawn

Answer 9

d. 450 mL

525 mL max

Question 10

E. permanent deferrals

Answer 10

e. positive for HBsAg

Question 11

F. weight

Answer 11

f. greater than or equal to 110

Question 12

G. deferral for malaria

Answer 12

g. one year

Question 13

H. Max body temp

Answer 13

37.5C or 99.5F

Question 14

Mom is R1r
child 1 is rr
child 2 is R2r

What is dad?

Answer 14

R2r

Question 15

What blood type is used for emergency release and alternate blood selection?

Answer 15

O neg

Question 16

Blood storage refrigerator temp

Answer 16

1-6 C

Question 17

Length of time recipient samples are stored

Answer 17

7 days

Question 18

Shelf life after hermetic seal is broke

Answer 18

24 hours

Question 19

Temp range during whole blood shipment

Answer 19

1-10 C

Question 20

Criteria of discarding blood units

Answer 20

hemolyzed

Question 21

What is the order of re-activity of ABO blood groups with anti-H?

Answer 21

O, A2, B, A2B, A1, A1B

Question 22

Hemphiliac patient would need what type of blood component?

Answer 22

Cryo

*contains concentrated fibrinogen and Factor VIII

Question 23

What is unique about the Lewis System?

Answer 23

• Only blood group system not manufactured by RBCs.
• Can show in saliva and tears
• Pregnant women may present Le(a-b-) and produce Lewis antibodies even though true phenotype is Le(a-b+)

Question 24

What antibodies are found in Bombay individuals?

Answer 24

anti-A, B, AB, H

Question 25

Panel study

Answer 25

choose 2 negative and 1 positive for both antibodies

Question 26

What blood group antigens are enhanced by enzymes?

Answer 26

``` Lewis Rh Kidd Ii P ```

Question 27

What blood group antigens are destroyed by enzymes?

Answer 27

Fya, Fya, MNS

Question 28

What would you expect if a Rh neg pt who has never been transufed or pregnant, was transfused with Rh pos blood?

Answer 28

No reaction; no antibodies are present b/c there has been no prior exposure

Question 29

Who should Rhogam be given? | When?

Answer 29

Rh neg mom, Rh pos baby | 28 weeks gestation, 72 hrs within delivery

Question 30

Used to remove Ab bound to sensitized rbcs; determine alloAb, autoAb, or HDN

Answer 30

elution

Question 31

using serum antibodies to detect unknown antigens detect presence or absence of Ag

Answer 31

Forward typing

Question 32

In vivo used to detect Ab attached to pt rbc when +, use elution to remove Ab to ID specific eluate used to diagnose HDN, HTP, AIMA

Answer 32

DAT

Question 33

Anti-IgG and anti C3d

Answer 33

polyspecific AHG

Question 34

Anti-A1 lectin is used to detect what?

Answer 34

A1 subgroups | - ABO discrepancy -

Question 35

Found on cord blood

Answer 35

Wharton's jelly

Question 36

Why are reverse typings done on newborns?

Answer 36

Antibodies are not yet developed

Question 37

chills, fever, increase temp by 2C

Answer 37

febrile reaction

Question 38

What could a weak subgroup of A cause?

Answer 38

ABO discrepancy

Question 39

What would you use to distinguish between an A1/A2 discrepancy?

Answer 39

Dolichus Biflorus

Question 40

If mom is O and the baby is A and there is HDN, what would you transfuse with?

Answer 40

O

Question 41

Would A2B with anti-A1 cause a discrepancy?

Answer 41

Yes

Question 42

What would the next step be to ID the cause of a strong positive DAT in cord blood and mother sample?

Answer 42

perform an elution

Question 43

Why are the cells washed in the AHG test?

Answer 43

to prevent neutralization by globulins

Question 44

What is the reason for a mf reaction on a DAT for a pt who has been previously transfused?

Answer 44

2 cell populations in the patients blood

Question 45

What type of sample do you need for compatibility testing? | Why?

Answer 45

FRESH serum | to preserve complement

Question 46

What type of elution is required for HDN?

Answer 46

acid elution; if not ABO incompatibility

Question 47

ARIPLKidd

Answer 47

antibodies enhanced by enzymes

Question 48

MNS, Duffy, Xga

Answer 48

antibodies, destroyed, inactivated by enzymes Kell is not affected