Module 10 Flashcards
1
Q
What is required for PCR
A
2
Q
what composes a pcr reaction mixture and how can you determine # of copies over 3 cycles
A
3
Q
What are the cycle temps and times
A
4
Q
How does PCR keep going
A
5
Q
Describe Taq polymerase
A
6
Q
Describe Pfu polymerase
A
7
Q
Describe primer design rules
A
8
Q
Describe Tm and annealing temperature
A
9
Q
Describe reaction controls
A
10
Q
What are the different thermocyclers/PCR machines
A
-Basic: every tube cycles together
-Gradient: allows optimization of annealing temps
-Real time: allows quantification each cycle
11
Q
Describe CODIS markers and human identification
A
12
Q
Describe PCR in disease testing
A
13
Q
Describe mutagenesis in PCR
A
14
Q
Describe quantitative PCR
A
15
Q
Describe sequence assembly in PCR
A
