Module 9 Flashcards by Kris Kriel

Q

What are the types of plasmids

A

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Q

What are the two virulent plasmids in B. anthracis

A

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Q

What are cloning vectors and their functions

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Q

Describe the basic cycle of DNA (sub)cloning

A

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Q

Describe restriction/modification system of bacteria

A

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Q

Describe the types of restriction enzymes

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Q

Describe a generic digest reaction

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Q

What are the products of single and double digests

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Q

What is agarose gel electrophoresis

A

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Q

How can we visualize DNA

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Q

Describe sizing of the bands

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Q

What is the effect of agarose concentration

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Q

Explain restriction mapping

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Q

What are the problems of big fragments

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Q

What is pulse-field gel electrophoresis used for

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Q

What is pulsenet

A

Q

Describe ligation and transformation

A

Q

What is a multiple cloning site

A

Q

Describe plasmid extraction

A

Q

describe plasmid isolation (miniprep)

A

Q

What are common miniprep modifications/extras

A

Q

Describe chromosomal DNA isolation

A