Module 7 Flashcards

(18 cards)

1
Q

What is electrophoresis

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2
Q

What is protein gel electrophoresis

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3
Q

What is SDS-Page

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4
Q

What is acrylamide

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5
Q

Describe SDS-PAGE protein and gel preparation

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6
Q

Describe loading, running and visualizing the gel

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7
Q

What is the protocol of silver staining

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8
Q

How is protein sizing used for gel analysis

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9
Q

How can you fix overlapping bands

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10
Q

What are stacking gels

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11
Q

Describe the movement of Cl, Glycine and protein in the stacking and running portion in SDS Page

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12
Q

What are Urea denaturing gels

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13
Q

What are native (non-denaturing gels)

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14
Q

What is 2-dimensional protein electrophoresis

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15
Q

Describe the first dimension of 2D protein electrophoresis

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16
Q

Describe the second dimension of 2D protein electrophoresis

17
Q

What are the uses for 2D gels

18
Q

Describe band identification