Module 7 Flashcards
(18 cards)
What is electrophoresis
What is protein gel electrophoresis
What is SDS-Page
What is acrylamide
Describe SDS-PAGE protein and gel preparation
Describe loading, running and visualizing the gel
What is the protocol of silver staining
How is protein sizing used for gel analysis
How can you fix overlapping bands
What are stacking gels
Describe the movement of Cl, Glycine and protein in the stacking and running portion in SDS Page
What are Urea denaturing gels
What are native (non-denaturing gels)
What is 2-dimensional protein electrophoresis
Describe the first dimension of 2D protein electrophoresis
Describe the second dimension of 2D protein electrophoresis
What are the uses for 2D gels
Describe band identification