Module 2 Flashcards
Describe basic microscopy
Thickness of section: too thick can lose ability to discriminate structures
lighting: can generate more contrast by altering amount and angle of light
What is the anatomy of a microscope and how do you focus it
What is magnification and resolution?
Resolution is the distance two objects need to be apart in order for us to see in between them or the width of an object that it needs to be in order to make it visible
-a lense that is closer will have a larger light angle and thus better resolution
-Shorter wavelength = smaller R which equals higher resolution
-Oil immersion has higher refractive index than air providing better resolution
what is spectrophotometry
what is absorbance vs transmittance
Transmittance: the proportion of light that the sample allows to pass (usually %)
Absorbance: the amount of light the sample blocks, calculated as A = Log (1/T) OR -Log(T) values of 0-2 are typical
-if given abs to solve for T, take 10^-A
what is beers law?
What are direct spectrophotometric measurements
rely on compound interactions with light
What are bradford assays
What are biuret assays?
