Module 2 - Microscopy Annotations

Question 1

Ocular Lens

Answer 1

• Magnifies specimen (but does not resolve the image).
• Can be dismantled to insert an eye piece graticule to enable specimens to be measured accurately.
• Magnifies specimen by x10
• Produces the ‘real image’ observed by user.

Question 2

Body Tube

Answer 2

-Passes light rays from objective lens to ocular lens.

Question 3

Revolving Nose Piece

Answer 3

• Holds up to 4 objective lenses: enables objectives lenses to be changed to select different focal lengths to achieve different powers of magnification.
• Rotates to enable selection of objective lenses.

Question 4

Objective Lens

Answer 4

• Magnifies and resolves specimen.
• High power objective lens: magnifies by x100
• Low power objective lens: magnifies by x4

Question 5

Mechanical Stage

Answer 5

• Supports slide in correct position at 90 degrees to illumination source.
• Enables light rays to pass through specimen.

Question 6

Condenser

Answer 6

-Focuses light from illumination source onto specimen.

Question 7

Iris Diaphragm

Answer 7

• Controls intensity of light reaching specimen.

- Best definition achieved with lower light intensity.

Question 8

Substage Illumination

Answer 8

• Source of illumination.
• Usually white light in school laboratories.
• Blue light bulb can be used to use light of a shorter wavelength to improve resolution.

Question 9

Course Focus Adjustment

Answer 9

• Raises/lowers the mechanical stage.
• Makes larger scale adjustments to the focus of the specimen.
• Used to locate the specimen in the field of view.

Question 10

Fine Focus Adjustment

Answer 10

• Raises/lowers the mechanical stage.
• Makes small scale adjustments to the focus of the specimen.
• Used to bring image into focus and ensure sharp image is observed.

Question 11

Mirror

Answer 11

-Used to deflect light rays from external light source up through the diaphragm and condenser to pass through the specimen.

Question 12

Stage Clips

Answer 12

-Hold the slide in situ.

Question 13

Arm

Answer 13

Used to carry and transport LM in the field.