Module 2 Section 4 - Action of Enzymes Flashcards

1
Q

What do enzymes do

A

They speed up chemical reactions by acting as biological catalyst

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2
Q

Enzymes are ……

A

Biological catalysts

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3
Q

What is an example of enzymes being used at a cellular level

A

Respiration

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4
Q

What is an example of enzymes being used for organisms as a whole

A

Digestion in mammals

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5
Q

What do enzymes affect

A

Structures in an organism
Functions of an organism

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6
Q

Enzyme action can be ….

A

Intracellular and extracellular

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7
Q

What does intracellular mean

A

Within cells

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8
Q

What does extracellular mean

A

Outside cells

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9
Q

What is the toxic by product of many cellular reactions

A

Hydrogen Peroxide (H2O2)

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10
Q

What happens if hydrogen peroxide is left to build up

A

It kills cells

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11
Q

What is the enzyme responsible for the breakdown of H2O2

A

Catalase, hydrogen peroxide to harmless oxygen and water

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12
Q

Give an example of an intracellular enzyme

A

Catalase

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13
Q

Give an example of extracellular enzyme

A

Amylase and Trypsin

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14
Q

Where does catalase work

A

Inside the cell (intracellular)

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15
Q

Where does amylase and trypsin work

A

Outside cells (extracellular) in the digestive system

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16
Q

Where is amylase found

A

Saliva (secreted to the mouth by salivary glands)

17
Q

What does amylase catalyse

A

The hydrolysis of starch to maltose (a sugar) in the mouth

18
Q

What does trypsin catalyse

A

The hydrolysis of peptide bonds - turns big polypeptides to smaller ones

19
Q

Where is trypsin produced

A

Cells in pancreas

20
Q

Where is trypsin secreted to

A

Small intestine

21
Q

What type of protein are enzymes

A

Globular protein

22
Q

Enzymes have…

A

An active site, which has a specific shape

23
Q

What is the active site

A

The part of the enzyme that the substrate molecules bind to

24
Q

How is the specific shape of the active site determined

A

By the enzymes tertiary structure

25
What need to happen for the enzyme to work
Substrate has to fit into the active site.
26
What happens if the substrate doesn’t match the active site
If the substrate doesn’t match the active site the reaction won’t be catalysed. Enzymes work few substrates - usually one.
27
What is activation energy
A certain amount of energy that needs to be supplied to the chemicals before the reaction will start - often provided as heat.
28
What do enzymes do to the activation energy
Reduces activation energy - speeds up rate of reaction
29
What is formed when the substrate binds to an enzymes active site
Enzyme-substrate complex
30
The formation of the enzyme substrate complex lowers ….
The activation energy
31
Why does the formation of the enzyme-substrate complex lower the activation energy
-if two substrate molecules need to be joined, attaching to the enzyme holds them close together reducing any repulsion so they can bind easily -if the enzyme is catalysing a breakdown reaction fitting into the active site puts a strain on bonds in the substrate. This strains means the substrate molecule breaks more easily
32
Enzymes only work with
Substrates that fit their active site
33
What did early scientists come up with to study action of enzymes
Lock and key theory
34
What is the lock and key theory
This is where the substrate fits into the enzyme in the same way that a key fits into a lock
35
Why did scientists realise that lock and key wasn’t totally perfect
New evidence showed that enzyme-substrate complex changed shape slightly to complete the fit.