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Flashcards in Molecular Bio Deck (114)
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61

Rho activation promotes both the bundling of actin filaments with ___ __ filaments into stress fibers and the clustering of interns and associated proteins to form focal contacts

myosin II

62

Name the junction:
seals gap between epithelial cells

tight

63

Name the junction:
connects actin filament bundle in one cell with that in the next cell

adherens

64

Name the junction:
connects intermediate filaments in one cell to those in the next cell

desmosomes

65

Name the junction:
allows the passage of small water-soluble molecules from cell to cell

gap

66

Name the junction:
anchors intermediate filaments in a cell to extracellular matrix

hemidesmosome

67

In regards to adhesion proteins, ___ mediate cell-cell connection and ____ mediate attachment of cells to matrix

cadherin; integrin

68

Adherents junctions often form a continuous ___ ___ close beneath the apical face of the epithelium, encircling each of the interacting cells in the sheet

adhesion belt

69

Each sealing strand of tight junction is composed of a long row of ___ pass transmembrane adhesion proteins embedded in each of the two interacting plasma membranes

4

70

two main tight junction proteins are what?

claudin and occludin

71

Gap junction is spanned by channel forming protein called what?

connexin

note: 6 connexin protein subunits

72

Gap junctions couple cells both electrically and metabolically. True or false?

true

73

Intracellular linkage of integrin to actin is mediated by ___

talin

74

What are FAK molecules and what do they do?

cytoplasmic protein tyrosine kinase; they autophosphorylate each other and make docking sites for Src-family kinases

75

Describe the structure of a proteoglycan

long polysaccharide/core complex linked to extensive hyaluronic acid chain

76

describe the triple helix of collagen

each chain twists to left; twist helically around each other to the right

77

Procollagen triple helix is formed where?

ER/Golgi Complex

78

Procollagen is then cleaved outside of cell where it self-assembles into a what?

collagen fibril

aggregation of fibrils forms fiber

79

What is the defective enzyme in scurvy?

prolyl hydroxylase

80

What is the defective enzyme in ehlers-Danlos VI?

lysl hydroxylase

81

What is the purpose of doing an SDS-PAGE?

separating proteins by size

82

Why would you do Mass Spec?

identify unknown proteins

83

Why would you do a western blot?

to analyze specific proteins

84

Which ELISA is necessary for HIV test?

indirect

85

What is an example of a sandwich ELISA?

pregnancy test

86

Why would you use nuclear magnetic resonance?

to analyze protein structure

87

Plasmids are cloning vectors. true or false?

true

88

How do cDNA clones work?

DNA copy of mRNA; use reverse transcriptase

89

What is pcr used for?

to detect altered genes or foreign DNA

90

What does CSI utilize?

STRs