Flashcards in Molecular Bio Deck (114)
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61
Rho activation promotes both the bundling of actin filaments with ___ __ filaments into stress fibers and the clustering of interns and associated proteins to form focal contacts
myosin II
62
Name the junction:
seals gap between epithelial cells
tight
63
Name the junction:
connects actin filament bundle in one cell with that in the next cell
adherens
64
Name the junction:
connects intermediate filaments in one cell to those in the next cell
desmosomes
65
Name the junction:
allows the passage of small water-soluble molecules from cell to cell
gap
66
Name the junction:
anchors intermediate filaments in a cell to extracellular matrix
hemidesmosome
67
In regards to adhesion proteins, ___ mediate cell-cell connection and ____ mediate attachment of cells to matrix
cadherin; integrin
68
Adherents junctions often form a continuous ___ ___ close beneath the apical face of the epithelium, encircling each of the interacting cells in the sheet
adhesion belt
69
Each sealing strand of tight junction is composed of a long row of ___ pass transmembrane adhesion proteins embedded in each of the two interacting plasma membranes
4
70
two main tight junction proteins are what?
claudin and occludin
71
Gap junction is spanned by channel forming protein called what?
connexin
note: 6 connexin protein subunits
72
Gap junctions couple cells both electrically and metabolically. True or false?
true
73
Intracellular linkage of integrin to actin is mediated by ___
talin
74
What are FAK molecules and what do they do?
cytoplasmic protein tyrosine kinase; they autophosphorylate each other and make docking sites for Src-family kinases
75
Describe the structure of a proteoglycan
long polysaccharide/core complex linked to extensive hyaluronic acid chain
76
describe the triple helix of collagen
each chain twists to left; twist helically around each other to the right
77
Procollagen triple helix is formed where?
ER/Golgi Complex
78
Procollagen is then cleaved outside of cell where it self-assembles into a what?
collagen fibril
aggregation of fibrils forms fiber
79
What is the defective enzyme in scurvy?
prolyl hydroxylase
80
What is the defective enzyme in ehlers-Danlos VI?
lysl hydroxylase
81
What is the purpose of doing an SDS-PAGE?
separating proteins by size
82
Why would you do Mass Spec?
identify unknown proteins
83
Why would you do a western blot?
to analyze specific proteins
84
Which ELISA is necessary for HIV test?
indirect
85
What is an example of a sandwich ELISA?
pregnancy test
86
Why would you use nuclear magnetic resonance?
to analyze protein structure
87
Plasmids are cloning vectors. true or false?
true
88
How do cDNA clones work?
DNA copy of mRNA; use reverse transcriptase
89
What is pcr used for?
to detect altered genes or foreign DNA
90
