1
Q
What is a housekeeping gene and why do we need it?
A
Actin, GADPH
To show equal loading.
2
Q
Outline the Transcriptome.
A
All protein coding mRNAs in cell
Always changes (differentiation, extracellular signals etc)
Each cell receives part of parents transcriptome
cell maintains A transcriptome throughout life - snapshot
3
Q
How does differential screening and differential display differ?
A
Screening = probe cDNA library with labelled mRNA from normal & diseased.
Display = Total RNA used in PCR to amplify genes from 3’ end
different bands are picked, purified & cloned.
