NON-BLOOD SPECIMEN HANDLING AND PROCESSING

Question 1

BODY FLUIDS

Answer 1

Cerebrospinal Fluid (CSF)
Peritoneal Fluid
Pericardial Fluid
Pleural Fluid

Question 2

NON-BLOOD SAMPLES

Answer 2

BODY FLUIDS
URINE
STOOL
SPUTUM
SEMEN
OROPHARYNGEAL (OP)
NASOPHARYNGEAL (NP) SWAB NASOPHARYNGEAL WASH/ASPIRATE
TISSUES

Question 3

is the most common sample the patient needs to collect.

Answer 3

Urine

Question 4

Urine: for samples that will be used for culture, the container must be

Answer 4

sterile

Question 5

Urine: Collection by a _____ is the most common method.

Answer 5

patient

Question 6

is used to collect urine for younger children bec they do not have control of their bladder function.

Answer 6

Pediatric urine collection bag

Question 7

URINE TYPE:

Answer 7

Random
first morning
8-hour sample
timed sample
double voided
24-hour sample

Question 8

URINE COLLECTION:

Answer 8

Mid-stream
clean-catch
catheter (culture)

Question 9

URINE VOLUME

Answer 9

30 - 60ml

Question 10

URINE PRESERVATION:

Answer 10

Refrigerator Temp (2-6 degrees Celsius)

Question 11

URINE PROCESSING: (Time frame)

Answer 11

Within 2 hours

Question 12

URINE PROCESSING:

Answer 12

-Physical Examination (Color and Clarity)
-Chemical Examination (SG, pH, Glucose and Protein)
-Microscopic Examination (WBC, RBC, Bacteria, Epithelial Cells)

Question 13

SEMEN COLLECTION:

Answer 13

collected on-site

Question 14

SEMEN CONTAINER:

Answer 14

Sterile
Wide-mouth container

Question 15

SEMEN TEMPERATURE:

Answer 15

Must not be refrigerated but kept as close to body temperature

Question 16

SEMEN PROCESSING: ( Time frame)

Answer 16

Within 2 hours

Question 17

SEMEN PROCESSING:

Answer 17

-Volume
-Sperm count, movement and shape
White Blood Cells

Question 18

SPUTUM COLLECTION:

Answer 18

Deep respiratory secretions
not saliva

Question 19

SPUTUM VOLUME:

Answer 19

about 1 teaspoon (5 milliliters)

Question 20

SPUTUM PROCESSING: (Time frame)

Answer 20

Immediately

Question 21

SPUTUM PROCESSING:

Answer 21

-Gram Stain
-Sputum Culture
-AFB

Question 22

STOOL COLLECTION:

Answer 22

Freshly Collected

Question 23

STOOL VOLUME:

Answer 23

0.5 to 1.0 gram
pea-sized

Question 24

STOOL PROCESSING: (Time frame)

Answer 24

Within 2 hours

Question 25

STOOL PROCESSING:

Answer 25

-Physical Examination (Color and Consistency)
-Microscopic Examination (WBCs, RBCs, Parasite, Yeast)

Question 26

NASOPHARYNGEAL WASH/ASPIRATE

OPTIMAL TIMING:

Answer 26

Within 3 days and not later than 7 days

Question 27

NASOPHARYNGEAL WASH/ASPIRATE

SPECIMEN COLLECTION:

Answer 27

Insert tubing into the nostril parallel to the palate.

Question 28

NASOPHARYNGEAL WASH/ASPIRATE

SPECIMEN HANDLING:

Answer 28

Sterile vials @ 4 degrees Celsius

Question 29

OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS

OPTIMAL TIMING-

Answer 29

Within 3 days
No later than 7 days

Question 30

OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS

SWAB TYPE-

Answer 30

Sterile Dacron or rayon swabs with plastic shafts

Question 31

OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS

SPECIMEN HANDLING-

Answer 31

Immediately into a sterile vial with 2 mL of viral transport media w/o antibiotics

Question 32

OP COLLECTION-

Answer 32

Posterior pharynx and tonsillar areas

Question 33

NP COLLECTION-

Answer 33

Nares parallel to plate