Nucleic Acids Flashcards

(38 cards)

1
Q

monomers of nucleic acids

A

nucleotide

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2
Q

nucleotides made up of

A

phosphate group,
sugar,
nitrogenous base

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3
Q

charge of phosphate group in nucleotides

A

negative

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4
Q

sugar in nucleotides

A

5 carbons, deoxyribose and ribose (dna and rna)

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5
Q

nitrogenous bases (2)

A

pyramidines or purines

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6
Q

purine

A

larger, A & G

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7
Q

pyramidine

A

smaller, C, U, T

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8
Q

dna bases

A

ACGT

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9
Q

Rna bases

A

ACGU

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10
Q

what is the base connected to; bond name

A

1st carbon; n glycosidic bond

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11
Q

base + sugar

A

nucleoside

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12
Q

3 non-genetic functions of nucleotides

A

carry energy, coenzymes, signaling

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13
Q

chemical energy nucleotides carry

A

ATP

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14
Q

H used to think what held genetic info and why

A

protein (20 amino acids vs dna 4 nucleotides

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15
Q

H who used mice and bacteria, what kind

A

griffith; pneumonia

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16
Q

H griffith used which strain on the first mouse, outcome

A

S STRAIN of S pneumoniae, dies

17
Q

H, griffith, which strain has a sugar capsule and is it deadly

A

S strain & is deadly

18
Q

H griffith used this strain on 2nd mouse and outcome

A

R strain, lived

19
Q

H, griffith, third mouse

A

s strain heated, mouse lived

20
Q

H, griffith last mice

A

injected r and heated s strains and mouse died, extracted s from dead mouse and injected into new one & mouse died

21
Q

H, Griffith importance of study

A

realized something (genetic information) was being passed from cell to cell

22
Q

H 2nd group of people

A

avery and mcleod

23
Q

H what did avery and mcleod do

A

broke open capsules and separated biological parts

24
Q

H what did avery and mcleod use (tool) to separate

25
H what did avery and mcleod do to see results
added the parts to r-strain cells
26
H avery and mcleod conclusion and results of the last part of expiriment
DNA only changed the strain; conclusion: dna holds genetic info
27
H third people, used bacteriophage
*hershey* and chase
28
H bacteriophage
viruses attacking bacteria cell; most abundant biological entity
29
H what did hershey use to determine and mark to see which part infects
bacteriophage, radioactive isotopes- labeled cells
30
H, hershey, which parts labeled with what
Dna- 32P (has a phosphate group) | Protein- 35S (sulfur)
31
H, outcome of hersheys expiriment
only p (dna) was in cell, not s (protein)
32
H what three were involved in structure (picture)
franklin-took picture | crick and watson- used picture
33
H what did crick and watson do
realized the shape was a helix, then double helix
34
H chargaff
worked with the bases, recorded the percent of each in various species
35
on what basis does agarose gel electrophoresis separate molecules
size, shape, charge
36
explain migration according to charge
the pos/neg charge makes it move either way
37
why is glycerol added to the solutions before they are loaded into the wells
to weigh them down
38
2 nucleic acids
dna and rna