PAES 416:2002 Flashcards
(28 cards)
Agricultural Structures – Plant Tissue Culture Laboratory
PAES 416:2002
growth of protoplasts, cells, tissues, shoot tips, roots, anthers, embryos, flowers and
meristems in a laboratory medium
tissue culture
any nutrient material prepared for the growth and cultivation plant tissue
culture medium
process of making any material entirely free from living microorganism
sterilization
condition that describes the freedom of plant materials, culture medium, confines of the
culture vessel from contaminating microorganisms
asepsis
subjecting of cultures to conditions favorable to the growth of the plant tissue
incubation
flow air currents in which air currents do not intermingle
laminar flow
The building should be located away from ??? such as a gravel
driveway or parking lot, soil mixing area, pesticide storage, or dust and chemicals from
fields.
sources of contamination
The area immediately surrounding the building shall be ??? The access road, walkways and parking areas shall be
similarly treated.
concreted, covered with
asphalt or with similar material.
The site shall be accessible to
service roads, water supply and electric lines.
Structural Requirements
5.1 Roof
5.1.1 The roof structure should be ???
5.1.2 Roofing materials should be ???
5.1.3 Roof vents, when provided, shall be properly ???.
treated timber or steel with anti-rust paint.
G.I. sheet and other steel sheeting with corrosion
resistant coatings.
screened
Ceilings
5.2.1 Ceilings shall be at least ??? from the finished floor line.
5.2.2 Ceilings shall be constructed to prevent the ??? that might sift
through from the areas above or fall from overhead collecting surfaces onto equipment or
exposed products.
Ceilings shall be painted with ???
2.4 m
collection of dirt or dust
white emulsion or latex paint.
Wall
5.3.1 The wall shall be ???, smooth finished and properly painted. Internal surface of
the walls should be painted with ???
5.3.2 Wall in growth room shall be painted with ???.
5.3.3 All wall tops and ledges shall slope at ???
5.3.4 It should be coved to the floor-wall and at wall-to-wall junctions with a radius of
???
- concreted
- white emulsion or latex paint.
- antifungal-epoxic-paint
- 45°
- 50 mm - 60 mm.
Windows
5.4.1 All windows shall be properly installed with ??? screen.
5.4.2 Windowsill shall be at least ??? from the finish floor line.
- 16-mesh
- 1 m
Doors
5.5.1 Main doorways shall be at least ??? wide to allow easier movement of large
equipment and facilities (e.g. laminar flow cabinets, photo-thermal cabinets, and shelves).
Door for staff, storage and chemical rooms should be at least ???.
5.5.2 Doors and doorjambs shall be ??? material.
1.5 m
0.8 m
moisture and rust-resistant
Floor
5.6.1 Floor shall be ???, properly reinforced to prevent cracks and shall be smooth
finished or tiled.
5.6.2 Floor shall be ???. Cement floor shall be painted with ???.
5.6.3 The floor slope should be ???.
5.7 All other matters concerning structural design of the building not provided in this
Standard shall conform with the provisions of the ???
- concreted
- non-skid
- epoxy or urethane floor
enamel - 2% - 4% towards the drain
- National Structural Code of Building.
Glassware washing and storage area
6.1.1.1 The glassware washing area shall be located near the ???.
6.1.1.2 The glassware area shall be equipped with at least ???. The top of the working
space should be ???. Sink shall be ???
6.1.1.3 Adequate workspace should be provided on both sides of the sink; this space shall be
used for glassware soaking tubs and drainage trays.
6.1.1.4 Storage area shall be provided with ??? that are placed low enough to
allow easy access.
6.1.1.5 Storage cabinets/shelves for chemicals should be provided and it should be made of
non-corrosive materials
- sterilization and media
preparation areas - two sink
- tiled or smooth-finished
- stainless steel which is acid and alkali
resistant. - dust-proof cabinets
Media preparation and sterilization area
6.1.2.1 The area shall be provided with working tables. Tables should be up to ??? wide if
it is to be access from both sides, and should not exceed ??? if it is accessible only from
one side and the height should be ???. Tabletop shall be covered with
materials which are easily cleaned and which will stand disinfectant solution.
1.2 m
600 mm
850 mm – 900 mm
shall be provided for isolation of explants, initial culture and transfer
culture.
Transfer room
shall be provided to be used for all aseptic work.
Laminar flow hoods or still-air boxes
shall be isolated from the external environment. Anteroom should be
constructed to prevent the entry of contaminants.
6.3.2 Ceilings shall be insulated with a minimum ???
6.3.3 ??? should be provided for the production of photo-period sensitive
plants. For the workers, ??? should be provided.
6.3.4 Shelves or trays shall be provided. Shelves should be painted ??? and should be
made of ??? Shelves should be fitted with casters to
permit movement during cleaning and maintenance of the growth room.
6.3.5 The width of the shelves should be ??? if accessible only from one side and
should be ??? if accessible from both sides. The distance between each layer of the shelves
shall be ???.
6.3.6 Aisles and passage shall be at least ???.
- Growth room
- R-value of 2.64 (R-15).
- Artificial lighting
- 200 lux
- white
- wood, expanded metal or tempered glass.
- 405 mm
- 1 m
- 455 mm
- 1 m
Recommended lighting intensity for tissue culture laboratory
Area / Lighting intensity
lux (Lumen/m2)
Outside the laboratory / 100
General / 200
Working table / 500
Growth room / 100 - 1000
All electrical design and installation shall conform to
Philippine Electrical Code.
shall be provided to prevent temperature variations in the room from
exceeding the favorable culture environment
Thermostat
